The Shandong Academy of Agricultural Sciences has made new progress in the identification of key regulatory genes of peanut plant type

Recently, the Institute of Agricultural Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, published a report entitled "BSA-seq and genetic mapping identified candidate genes for branching habit in peanut" in Theoretical and Applied Genetics (Top/Q1, 2022 Impact Factor 5.
57).
".

The study reported AhLBA1, a key gene that controls peanut plant types, finely mapped the gene to the 136.
65 kb range of chromosome 15, and identified two candidate genes
.

 

Peanuts are an important oil crop and cash crop in China, and the development of flower production industry is of strategic significance
to ensure the security of grain and oil in China.
Reducing peanut production costs, increasing flower production, and realizing mechanized production are important topics for
peanut breeding and cultivation.
The plant type of peanuts directly affects the soil needle penetration, planting density, cultivation method and mechanization application
.
The lateral branch angle is the key
to determining the peanut plant type.
Compared with the upright type, creeping and semi-creeping peanuts can significantly reduce seed usage and reduce
the need for height control.
Creeping and semi-creeping varieties may be one of the
important directions for future peanut breeding.
Therefore, fine positioning and exploration of peanut lateral branch angle genes and molecular markers can provide theoretical basis and technical support
for peanut plant type improvement.

 

In this study, the creeping peanut variety Tifrunner was used as the mother and the erect peanut variety Fufrunner was crossed as the parent to construct the isolated population
.
BSA analysis was used to initially locate the main gene AhLBA1, which regulates the angle of peanut lateral branches, in the range of 150-160 m on
chromosome 15.
Indel and SNP markers were then developed in the candidate intervals to finely map the gene to a range of 1.
12 cM, corresponding to a physical interval
of about 136 kb on chromosome 15.
There are nine candidate genes in this interval, among which F-box protein PP2-A13 and 2-Oxoglutarate (2OG) and Fe(II)-dependent oxygenase are predicted to be the key genes controlling peanut lateral branch angle, and the results lay a foundation
for further revealing the molecular mechanism of peanut lateral branch angle regulation.
At the same time, the KASP and InDel molecular markers closely linked to the lateral branch angle of peanuts were also reported, which provided technical support
for the molecular marker-assisted selection of improved peanut plant types.

 

Associate researcher Pan Jiaowen and graduated master student Zhou Ximeng of the Institute of Germplasm Resources are the co-first authors of the paper, researcher Wang Xingjun and researcher Zhao Chuanzhi are the co-corresponding authors, and the Institute of Agricultural Germplasm Resources, Shandong Academy of Agricultural Sciences is the first
paper.
Shandong Normal University, Shandong College of Agricultural Engineering, Guangxi Academy of Agricultural Sciences, Henan Academy of Agricultural Sciences, International Institute of Tropical Semi-Arid Crops and Murdoch University of Australia participated in the research, which was supported
by the National Natural Science Foundation of China, Shandong Agricultural Seed Project, Shandong Academy of Agricultural Sciences Innovation Project, and Shandong Taishan Scholar Talent Project.