The structural function mechanism of the new argon/anti-anti-rhypby factor of special bacteria is revealed.

Transcription is the process by which RNA polymerases synthesize messenger RNA based on the DNA sequence of genes, and is the initial step in gene expression.
in bacteria, the argon factor is a key component for RNA polymerase to identify gene promoters and initiate transcription.
in recent years, a widely present class of xenon factors and their co-transcription anti-hermitiating factors, SigI and RsgI, have been found in some Clostridium difficile and bacteria, and some of their domains and known proteins have no homologous origin, representing a new class of special bacterial argon/anti-rhymo factors. some fibrous-producing bacteria, such as
, have 8-16 pairs of SigI/RsgI factors, which are rare in other known types of xenon/anti-pyridox.
existing studies have shown that these SigI/RsgI factors are responsible for the regulatory expression of fibrous small bodies, but their structure and functional mechanisms are still not clarified.
recently, Feng Yingang, a researcher in the institute of metabolites at Qingdao Energy Institute, led researchers in the study of the structural function mechanism of SigI/RsgI, a fiber-small body regulatory factor of clostridium difficile, and the relevant results were published in the influential international journal Nucleic Acids Research (the latest impact factor of the journal is 11.5).
research progress fiber small body is a multi-enzyme complex secreted by some anaerobic Clostridium bacteria, is an efficient lignocellulose degradation molecular machine, in the degradation of ligcellulose resources and biotechnology development has important application value.
the enzyme components of fiber small body are regulated by the type of extracellular substrate, and the study on the regulation mechanism of fiber small body is of great value to the understanding of the efficient mechanism of fiber small body and the application and development of fiber small body.
the institute of metabolites research group for many years to the research and application of Clostridium difficile and its fibrosis, using the research group's independent research group of genetic operating hardware equipment and software tools, the thermofidobacteria physiological biochemistry, the assembly of fiber small body assembly, synthesis regulation and product inhibition, product intake and metabolism, such as the systematic research.
to reveal the mechanism of SigI/RsgI's role in the regulation of fibrous small bodies, the researchers first clarified the combination of RsgI and SigI through mrivitive experiments, and further analyzed the three-dimensional structure of the complex.
results show that RsgI is mainly formed by combining the intracellular domain with sigI's C-end domain, wherein The intracellular domain of RsgI is a barrel structure composed of beta chips, and the C end of SigI is formed by 8 alpha spirals, and a stable complex is formed by a variety of forces.
this compound structure is completely different from other known argon/anti-anti-factor complex structures and represents a unique type of argon/anti-anti-factor complex structure.
the researchers further revealed the key regions of DNA on SigI to identify promoter-35 regions, and found that the identification specificity between SigI/RsgI factors was achieved through synergy between multi-pair residues on two proteins.
these results reveal the structural function mechanism of this new type of argon/anti-rhybo factor, which makes people understand more about the process of transcription of bacteria and the molecular mechanism of extracellular environment induction, and also helps to improve people's understanding of the mechanism of regulation of fibrous small body, which can provide a basis for the transformation and application of fiber-producing small body bacteria.
in addition, the argon/anti-rhion factor is an important regulatory element in synthetic biology research, and the new argon/anti-rheutium factor can provide more selectivity for the development of synthetic biology elements, and an understanding of their mechanisms will help improve the performance of these components.
research results were supported by the National Natural Science Foundation of China, the Chinese Academy of Sciences and Shandong Province.
the completion of the study by researchers from the Weizmann Institute of Science in Israel and Tel Aviv University, supported by the NSFC's China-Israel International Cooperation Exchange Project.
the collection of some high-field MRI data in this study was greatly assisted by Dr. Yao Hongwei of Xiamen University's High Field Nuclear Magnetic Resonance Center.
Wei Zhen, a doctoral student at Qingdao Energy Institute, is the first author of the research paper, Feng Yingang is the communication author of the paper, and a number of students and researchers from the atabmatic group are involved in the research work of the project.
Figure 1. The composite structure of the new argon/anti-xenon factor SigI/RsgI is completely different from that of other known argon/anti-rheutium factors.
left is the structure of SigI/RsgI, and the figure on the right is the structure of three other known anti-rhyun/anti-xenon factor complexes.
Source: Qingdao Institute of Bioenergy and Process, Chinese Academy of Sciences.