The study revealed the molecular mechanisms of salt resistance differences between pumpkins and cucumbers

pumpkins are more salt-resistant than cucumbersRecently, the Journal of Experimental Botany published a research paper online on the team work of Professor Berlong of Huahua Agricultural University and Professor Sergey Shabala of the University of Tasmania, Australia. This paper analyzes the important molecular physiological mechanism of pumpkins with strong salt resistance than cucumbers, mainly because pumpkin root tip RBOHD produces hydrogen peroxide (H2O2) signal under salt stress, regulates potassium absorption through the mass membrane H-ATPase, and then affects the vitality and salt resistance of pumpkin root cells, while the regulation of cucumber potassium absorption is not obvious.
pumpkin is more salt-resistant than cucumbers, is an important cucumber birch, the cucumber grafted to the pumpkin birch can significantly improve salt resistance. However, the molecular physiological mechanism of salt resistance differences between pumpkins and cucumbers has been unclear. H2O2, as an important reactive oxygen signal molecule, is involved in the process of growth and development and resistance regulation of many plants.
The study found that pumpkins were more salt-resistant than cucumbers, and that pumpkin root tip had higher potassium absorption rate, potassium content and H2O2 levels, using cell dynamic dyeing, non-injury ion selective microelectrectos (MIFE), potassium content determination, H2O2 fluorescence imaging, and so on. The transcription group found that 85 percent of the difference genes at the root of pumpkins were significantly increased, and only 52 percent of the difference genes in cucumbers were significantly increased. Salt-stressed pumpkin NADPH oxidase, 14-3-3 protein, mass membrane H-ATPase and potassium transport-related genes RBOHD, GRF12, AHA1 and HAK5 significantly increased expression, but there was no significant change in cucumbers.
the material root system was pre-treated with NADPH oxidase inhibitor DPI, and it was found that the formation of pumpkin root H2O2 was blocked after adding inhibitor DPI, GRF12, AHA1, HAK5 expression, mass membrane H-ATPase activity and potassium absorption rate decreased significantly, and the exogenous H2O2 treatment resulted in the opposite result. Further using CRISPR/Cas9 gene editing and amoeba genetically modified means, the H2O2 signal produced by pumpkin RBOHD was verified to regulate the molecular physiological mechanism of potassium absorption and salt resistance through the mass membrane H-ATPase. (
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