echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > The study reveals a new mechanism for chromatin modification to regulate plant gene expression.

    The study reveals a new mechanism for chromatin modification to regulate plant gene expression.

    • Last Update: 2020-08-08
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    August 6, the Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences/ Plant Physiological Ecology Research Center Plant Adversity Biology Research Center Plant Molecular Genetics National Laboratory He Yuehui Research Group (in cooperation with Liu Renyi Research Group) and Du Jiamu Research Group (in cooperation with the Bell Snowflake Research Group of the University of Wisconsin, USA) "Nature-Genetics" back-to-back published polycomb-mediadgene silencing by the BAH-EMF1 complex plants and EBS is a bivalent histone that the reader euthane sphin transition in Arabidopsis.
    two articles using biochemical, molecular, genetic, histological and structural biology research methods, respectively, revealed the plant-specific chromatin coagulation protein EMF1 and THE BAH-containing domain of SHL and EBS to form BAH-EMF1 complex and mediate plant gene silencing the molecular mechanism, as well as the difycolor protein marker reader EBS in the preparation of the southern mustard flowering time regulation of the role mechanism. The methylation modification of
    histone lysine plays a wide role in the gene expression regulation of enucleosome organisms and is an important mode of regulation.
    the trimethylation (H3K4me3) modification of histone H3, the 4th lysine, is usually associated with gene activity expression, while H3's 27th lysine trimethylatization (H3K27me3) modification is associated with antagonizing and inhibiting gene expression.
    H3K27me3 retouching is mediated by the Polycomb Protein Family (PcG).
    multicellular organisms in the cell differentiation process, some genes are silenced by the PcG protein to maintain the properties of the cells after differentiation (cell identity).
    PcG protein setypes form two protein complexes, PRC1 and PRC2, which inhibit the transcription of target genes through chromatin modification.
    the various components of PRC2 are highly conservative in plants and animals, and its molecular function is catalyzed H3K27me3.
    in animals, H3K27me3 is identified and combined by the componentPc in the PRC1 complex, which in turn inhibits gene expression through the chromatin coagulation mediated by another component, Ph.
    plants do not have pc and Ph homologous proteins, but H3K27me3 is a modified retouching in the genome, and how plants decrypt the silent marker of H3K27me3 is unclear.
    previously found in the pattern seed plant amoeba LHP1 protein can identify H3K27me3, however lhp1 missing mutant phenotype weak, less affected genes, thus suggesting that there are other H3K27me3 identification proteins in the plant and plant-specific chromatic coagulation protein EMF1 synergetic inhibition gene expression.
    , as a result, plants appear to have evolved a different H3K27me3 decryption mechanism from animals to regulate gene expression.
    He Yuehui's research group found the do-soprotein SHL and EBS of the amoeba EMF1 protein through protein interaction analysis.
    these two homologous proteins contain two domains, BAH and PHD.
    biochemical experiments show that the BAH domain not only mediates the interaction with EMF1, but also identifies H3K27me3, and further molecular genetic analysis has found that SHL and EBS and EMF1 form the "reader-effector" complex of H3K27me3 (SHL or EBS as reader, chromatin coagulation protein EMF1 is effector).
    the complex declassified the H3K27me3 silent marker on the genome and inhibited the expression of the target gene.
    in the three defective mutants of shl ebs lhp1, H3K27me3 on the amoeba genome could not be maintained, and the body cell differentiation of the seedlings was reversed to form the injured tissue;
    , the researchers found that in monolobite plant rice, the SHL-EBS family protein was able to identify H3K27me3 and interact with EMF1's homologous protein, forming a similar BAH-EMF1 "reader-effector" complex.
    these findings reveal that plants have evolved different H3K27me3 decryption mechanisms from animals over a long evolutionary period to inhibit gene expression and regulate growth and development.
    , in collaboration with the Dujiamu Research Group and the Bell Snowflake Research Group, found that EBS proteins are divalent histone marker readers: BAH and PHD domains identify H3K27me3 and H3K4me3 markers, respectively.
    in vitro experiments found that EBS combined with H3K27me3's affinity is higher than the affinity combined with H3K4me3, and further structural biology studies have found that EBS's BAH domain achieves the specificity of the selection of methylation lysine and 30th stenonine sequence by identifying the peptide segment H3K27me3.
    EBS can inhibit the PHD domain in combination with H3K4me3 by a irregular structure containing proline at the C end, in a self-inhibited manner.
    experiments in plants showed that EBS can bind H3K4me3 and H3K27me3 to chromatin, and its distribution in the genome of amoeba is similar to that of H3K27 methylaterase CLF.
    EBS inhibits the expression of the anthocyanin gene, thereby inhibiting flowering, further analysis found that EBS as a molecular switch, can identify H3K4me3 and H3K27me3 markers and precisely change their binding preferences to ensure timely flowering.
    these two studies reveal the new mechanism of plant cleverly decrypting the labels on target gene chromatin, to accurately regulate the expression of key genes, which is of great theoretical significance for understanding the basic scientific problem of plant gene expression regulation, and also provides a new way of thinking for the production and application of crop flowering regulation.
    .
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.