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    Home > Biochemistry News > Biotechnology News > The synthesis of the secondary wall of rice was created by CRISPR/Cas9 gene editing technology using CRISPR/Cas9 gene editing technology.

    The synthesis of the secondary wall of rice was created by CRISPR/Cas9 gene editing technology using CRISPR/Cas9 gene editing technology.

    • Last Update: 2020-08-19
    • Source: Internet
    • Author: User
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    Secondary wall is the most abundant renewable resources on the earth, natural secondary wall is often produced into a variety of fiber products, to serve people's daily life, can also be used as a paper industry and bioenergy raw materials, has important economic value.
    secondary wall is the material basis of plant growth and many physiological processes that affect life activities.
    for example, the level and quality of secondary wall synthesis in rice is directly related to the high plant, anti-fall ability and other important agronomic traits, so the synthesis is strictly regulated.
    study found that a large number of NAC, MYB and other types of transcription factors constitute a complex network to respond to various signals inside and outside the plant, accurate regulation of secondary wall biosynthesis.
    however, very few negative regulatory factors have been identified so far.
    The Seinleaf Angle1 (ILA1) is the previously reported Raf-like MAPKKK protein formed by the regulatory secondary wall, and the mutual protein ILA1 interacting protein4 (IIP4) is its phosphorylation substrate, and its biological function is unknown.
    the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, Zhou Weihua research team used CRISPR/Cas9 gene editing technology to create the iip4 mutant, found that its mutant secondary wall thickness increased significantly, cellulose and lignin content slightly increased, indicating the iIP4 negative regulation of the synthesis of secondary walls of rice.
    biochemical and molecular biology studies found that IIP4 and the top key regulatory factor nac29/NAC31 of secondary wall synthesis interacted to inhibit the expression of its downstream regulatory genes, such as MYB61, CESA4, CESA7 and CESA9, thus interfering with secondary wall synthesis.
    is altered by ilA1 phosphorylation of IIP4 subcellular positioning, transferred from the nucleus to cytoplasm, resulting in the release of the interchangeal protein NAC29/NAC31 in the nucleus, which promotes the expression of downstream genes and secondary wall synthesis.
    sequence analysis of IIP4, it is found that it has atypical CCCH base sequence (C-X4-C-X10-C-X2-H) with regular repetition.
    IIP4 homologous protein is widely present and highly conservative in plants, suggesting the importance of its function.
    IIP4's regulatory effect on secondary wall synthesis can be used to improve crop resistance to fall- and fall.
    this study analyzes the signal transduction pathway of ILA1-IIP, which provides an important basis for clarifying the molecular mechanism of the formation of secondary walls of rice and the design and breeding of high-yielding high-quality molecular sass of crops. The findings were published online November 21,
    in the journal Molecular Plant.
    research work is funded by the Natural Science Foundation project, the Chinese Academy of Sciences strategic leading science and technology special, the special project of genetically modified and the Youth Promotion Association.
    .
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