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On April 26, the international academic journal Circulation published the results of the Research Group of Zhou Bin of the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences: Genetic Lineage Tracing of Non-myocyte Population by Dual Recombinases.
the research using the newly established dual homologous recombination technology system to reveal the potential of non-muscle cell differentiation to form muscle cells during embryonic heart development, adult cardiac stabilization and damage repair, adult skeletal muscle stabilization and damage repair.
the results of this research can provide insight into the trans-differentiation of non-muscle cells in different organs, or at different stages of development of the same organ, and provide new ideas for the study of muscle regeneration.
The technology does not rely on specific stem cell molecular markers to study whether stem cells differentiate into functional cells such as muscle cells in tissues.
heart as one of the most important organs of vertebrates, the main function is to provide power for blood flow.
myocardial infarction causes a large number of deaths of myocardial cells and affects heart function.
the existence of myocardial stem cells in adult hearts has been debated, and previous studies have used traditional genetic lineage tracing techniques to suggest the presence of heart muscle stem cells in adult hearts, such as Kit-Heart Muscle Stem Cells, Bmi1-Heart Muscle Stem Cells, Scal1-Cardiomyopathy Stem Cells, Islet-Heart Muscle Stem Cells, etc., but because the molecular markers of these heart muscle stem cells are expressed in some heart muscle cells themselves, the potential for differentiation of these hypothetical heart muscle cells has been called into question.
in the study, researchers used double homologous recombination techniques to simultaneously trace muscle cells and non-muscle cells, systematically revealing the potential of non-muscle cells to differentiate into muscle cells.
early in embryonic heart development, Isl1 plus heart muscle stem cells can contribute to the second heart region of the heart muscle cells, such as the outflow of the heart, the right ventricle part of the heart muscle cells, and a very small number of myocardial cells in the left ventricle and the atrium are derived from Isl1 plus myocardial stem cells.
researchers used double homologous recombination techniques to specifically trace early embryonic non-cardiomyopathy cells to find that in early embryonic heart development, non-cardiomyocytes have the ability to differentiate to form cardiomyopathy cells, which can contribute to the formation of heart muscle cells in the second heart region.
in the steady state maintenance of the adult heart, the researchers used double homologous recombination technology to trace the simultaneous lineage of non-muscle cells and muscle cells, non-muscle cells and muscle cells maintain their own cell destiny, non-muscle cells do not differentiate to form muscle cells, and after the adult heart myocardial infarction, resulting in Heart muscle cells die in large numbers and heart function is impaired, but muscle cells and non-muscle cells in the heart still maintain their own cell destiny, non-muscle cells do not differentiate to form muscle cells to compensate for the death of muscle cells, that is, the adult heart in the steady state maintenance and repair of damage will not occur non-muscle cells to muscle cells in the process of rotation differentiation.
skeletal muscle in the steady state maintenance process, fibroblasts and non-muscle cells fusion, fibroblasts in a polynuclear state, and when the skeletal muscle is performed BaCl2 injury, a large number of fibroblasts in the skeletal muscle died, but during the repair of skeletal muscle damage, the researchers found that non-muscle cells in the skeletal muscle can be differentiated to form muscle fibrosis cells, promoting the repair of skeletal muscle damage.
the above research results will provide a theoretical basis for muscle regenerative medicine.
the research was carried out under the guidance of researcher Zhou Bin, ph.D. student Li Yan and associate researcher He Lingxuan.
the research work has received strong support from Professor Lu Ailan of the Chinese University of Hong Kong, and also received financial support from the Chinese Academy of Sciences, the State Fund Committee, the Ministry of Science and Technology, and the Shanghai Science and Technology Commission.
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