The team of Professor Gao Feng and Professor Xianghui Yu isolated anti-HIV-1 broad-spectrum neutralizing antibodies from a rhesus monkey model, and revealed that such antibodies mature into...

The research team of Professor Gao Feng and Professor Yu Xianghui from the School of Life Sciences of Jilin University took the rhesus monkey animal model with the longest reported infection time as the research object, and analyzed its broad-spectrum neutralizing activity induced in vivo

With the maturity of antibody isolation technology and the in-depth understanding of the mechanism of antibody - antigen evolution, a large number of broad-spectrum neutralizing antibodies against HIV-1

Figure 1

And through the cooperation with Professor Zhou Tongqing of the National Institutes of Health Vaccine Research Center, the comprehensive use of cryo-electron microscopy crystal structure analysis, homology modeling and virus single-genome sequencing technology to systematically study the J038 lineage broad-spectrum neutralizing antibody in SHIV - infected rhesus monkeys J038 antibody was found to bind to the special " upright " V2 loop of Env , making the broad-spectrum neutralizing antibody more likely to bind to the CD4 -binding region on Env ( Figure 2 ), and revealed the generation and evolution mechanism of a new V2 -specific broad-spectrum neutralizing antibody, that is, the broad-spectrum of such broad-spectrum neutralizing antibodies is in the process of evolutionary maturation through the continuous increase in the envelope The binding area of ​​the protein and its surface polysaccharides was obtained (Fig.

Figure 2

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This research work has been supported by the National Key R&D Program ( 2021YFC2301500 ), the National Natural Science Foundation of China ( 3197088882002138 ), the National Science and Technology Pillar Program during the " 13th Five-Year Plan " period ( 2018ZX10731101-001-010 and 2018ZX10731101-002-003 ), etc.

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