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The team of Professor Li Guiying and Associate Professor Yang Fang of the Key Laboratory of Molecular Enzymatic Engineering of the Ministry of Education, College of Life Sciences, Jilin University, have made important progress in the field of microfluidic liquid biopsy, and the relevant research content has recently been titled Immunofluorescence analysis of breast cancer biomarkers using antibody-conjugated microbeads embedded in a Microfluidic-based liquid biopsy chip Published online in Biosensors & Bioelectronics, 2022, 216: 114598
.
The early diagnosis, treatment and efficacy evaluation of diseases are of great significance
for human health monitoring and clinical diagnosis and treatment of diseases.
Liquid biopsy is a technique
for diagnosing and monitoring diseases by sampling and analyzing samples of a patient's body fluids (blood, urine, etc.
).
Compared with traditional tissue biopsy, microfluidic liquid biopsy has the advantages of low sample consumption, high throughput, convenient detection, detectable disease types, safety, non-invasive and early disease detection, and has broad application prospects
in early diagnosis of cancer.
However, because the liquid flow in the microchannel is usually laminar (i.
e.
, the liquid flows in layers and does not mix with each other), the mass transfer efficiency is low, resulting in a decrease
in the efficiency and sensitivity of biosensing.
In view of this, the team developed a continuous, rapid, and efficient immunofluorescence analysis-based liquid biopsy chip that disrupts laminar flow and improves mass transfer efficiency
by filling antibody-coated beads into the chip.
At the same time, the filled beads increase the contact area of the immune affinity reaction, greatly enhance the binding of antibodies to the target protein, and amplify the fluorescence signal, which significantly improves the sensitivity and efficiency
of the detection.
This microfluidic-based liquid biopsy device requires only a small amount of plasma sample (20-50 μL) to achieve rapid detection (55-75 min)
with low detection limits (LOD, 0.
1 ng/mL).
The team also tested the expression of breast cancer biomarkers carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3) in plasma samples from breast cancer patients and healthy volunteers, and the results showed that the method could accurately distinguish between plasma samples from cancer patients and healthy volunteers with high diagnostic accuracy
.
The study provides a new strategy
for rapid early diagnosis of cancer and other diseases.
Schematic diagram of microfluidic chip detection
(A) Microfluidic chip detection principle; (B) and (C) chip design drawings; (D) Physical diagram of the chip; (E) Microscopic antibody bead filling effect
The first author is Li Na, a master student from the College of Life Sciences, Jilin University, and Professor Li Guiying and Associate Professor Yang Fang of the Key Laboratory of Molecular Enzymatic Engineering, Ministry of Education
, College of Life Sciences, Jilin University.
This research was supported
by the National Natural Science Foundation of China, the Science and Technology Department of Jilin Province, and the Interdisciplinary Integration and Innovation Program of Jilin University.
Original link: https://doi.
org/10.
1016/j.
bios.
2022.
114598