The terrance photometric method determines the content of clerrel

, the experimental purpose
1. To understand the distribution
properties
the middle-
of the plant tissue.
2. Master the principles and methods for determining the content of yelorotin., experimental principle
Serphylorin is widely found in green plant tissues such as fruits and vegetables, and in plant cells with
protein
combined into serrogen. When plant cells die, chlorophyl is free, free chlorophyte is very unstable, sensitive to light and heat, chlorophyl produces green-brown demagum chlorophyl under acidic conditions, can be hydrolysed into bright green chlorophytes and chlorophyl and methanol in the thin lye. There are two types of chlorophytes in higher plants: chlorophytes a and b, both of which are soluble in ethanol, ether, acetone and chloroform.
the content of serotonin has a variety of methods, including:
1. Atomic absorption
spectral
method: by measuring the content of magnesium, and then indirectly calculate the content of chloroerra.
2. Hydratum method: using the
-hydrometer
to determine the absorption value of chloroelin extract at the maximum absorption wavelength, the content of the various pigments in the extract can be calculated using Lamber-Bill's law.
a and curlyror b have maximum absorption at 645nm and 663nm, and the two absorption curves intersect at 652nm. Therefore, the absorbent value of the extract at 645nm, 663nm and 652nm wavelengths is determined, and the contents of colorin a, serenity b and total curch can be calculated according to empirical formula.III, instruments, raw materials and
-reagents
instruments: hydrometers, electronic top-loaded
balance
(sensitivity 0.01g), research, brown
capacity bottle
, small
funnel
, quantitative filter paper, water-absorbing paper, wipe paper, titration.
Ingredients: Fresh (or dried) plant leaves
Reagents: 1.96% ethanol(or 80% acetone
); Called to take the shredded fresh sample 2g, put into the research, add a small amount of quartz sand and calcium carbonate powder and 3mL95% ethanol, into a total pulp, and then add ethanol 10mL, continue to grind until the tissue whitening. Set aside 3 to 5min.
Take filter paper 1 sheet placed in the funnel, wet with ethanol, along the glass rod to pour the extract into the funnel, filter flow to 100mL brown capacity bottle;
ethanol with a dropper and wash all the chlorophylic pigments on the filter paper into the capacity bottle. Until there is no green in the filter paper and residue. Finally, use ethanol to 100mL, shake well.
chroma pigment extract was used to measure absorbent at wavelengths 665nm, 645nm and 652nm, with 95% ethanol as a blank control., the calculation
according to the experimental principles provided in the emphysic formula, respectively, to calculate the plant materials in the content of clericin a, b and total