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    Home > Biochemistry News > Plant Extracts News > The thin-walled tissue and thick-angled tissue of the celery leaf handle

    The thin-walled tissue and thick-angled tissue of the celery leaf handle

    • Last Update: 2021-01-07
    • Source: Internet
    • Author: User
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    the thin-walled
    tissues
    and thick-angled tissues of celery leaf handles take the celery (Apium graveolens) leaf handle, cut it into small pieces with an anatomical knife or blade, and flatten the face you want to cut. Then take a sharp razor or a new blade and make a
    with
    . Move the sliced slice from the slicer to a water-filled
    culture
    dish, or place it directly in a droplet on the slide, which can be viewed with a covered slide.cells in celery leaf handles are larger than other cells, have thin cell walls, and have developed cell gaps. Because the cell gap in the fresh material is filled with air, it is often
    when observed under
    microscope. These cells are generally isomethortes (hetetrotes). Because the freehand slices are thick, the three-dimensional shape of these cells can be seen on the slices.can be dyed in order to see clearly. Commonly used dyes are 1% medyl blue, 0. 5-1% neutral red or 1% red water solution. Stained slices can be placed in 10% glycelycen, the method is to dye the water around the slice with absorbent paper, and then add 1-2 drops of 10% glycelycerne, after covering the slide can be observed. Glycer oil increases transparency and prevents the slice from dehydration and drying out, so it can be stored for a longer period of time. But glycelin must be neutral, otherwise it will fade the slice.on the cross-sectional surface of the celery leaf handle, you can also see the thick-angled tissue distributed in the corners protruding from the outside of the leaf handle. Thick-angled tissue cells can be identified according to two characteristics: one is that their cells have pearl walls, which are easily distinguished from other cells under a microscope, and the other is that the cell walls thicken at the corners and look like a one-star mango-like structure. One of the gray "caves" is the cell cavity, which is filled with protosites. The cell structure is then observed in a high-height object lens. After observation, the slice is treated with inter-phenol and hydrochloric acid, the first use of tweezers to remove the cover glass, add a drop of 1mol/L hydrochloric acid, after 1-2 minutes and then add a drop of inter-phenol, cover the cover glass, with absorbent paper to absorb excess liquid, you can observe under the microscope. When color reactions are not visible, their cell walls are thickened but not woody.。
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