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    Home > Medical News > Medical Research Articles > The three details are well grasped, and the success rate of human iPS-liver differentiation is up up up!

    The three details are well grasped, and the success rate of human iPS-liver differentiation is up up up!

    • Last Update: 2021-08-17
    • Source: Internet
    • Author: User
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    The importance of the liver is self-evident.
    There are more than 500 kinds of biochemical reactions in the liver.
    Once liver function is disordered, it will lead to a series of metabolic diseases

    .
    Whether you are engaged in the study of liver disease mechanism or liver-related drug metabolism, hepatocyte models with predictive functions are necessary tools

    .

    Hepatocytes differentiated from human iPS cells have similar functions to primary hepatocytes and retain the genetic background of the donor.
    It is a new type of hepatocyte model

    .
    The biggest bright spot is that iPS cells can proliferate steadily and can supply hepatocytes of the same genetic background as long as they continue to differentiate, avoiding the shortcomings of limited supply of human primary hepatocyte donor materials

    .

    Takara Cellartis iPS Cell to Hepatocyte Differentiation System (Y30055) is a complete, efficient and novice-friendly differentiation system that can generate functional human iPS-derived hepatocytes within three weeks
    .

    Come on, video! In 6 minutes, quickly learn about Takara's iPS-liver differentiation system (Y30055) !

    The essence of the details is a serious attitude and a scientific spirit, and the mystery lies in the details
    .
    The manual of Takara iPS-Hepatic Differentiation System (Y30055) has 29 pages, which can be said to be very detailed! The caring editor has consciously set the key points for everyone in the incarnation class representative.
    The following three details have been carefully selected, and the Takara liver differentiation system can be used efficiently, and the success rate will be up and up!

    1.
    Cell count

    Using the right seeding density is the key to successful iPS-liver differentiation, and it is important to count the cells correctly
    .
    We recommend using a hemocytometer
    .
    If you use an automated cell counter, you need to add the same cell suspension to the hemocytometer at the same time to verify the results of the automatic counting, so as to adjust the settings of the automatic counter accordingly

    .

    In the differentiation stage of iPS-DE cells , too sparse initial iPS cells will lead to a large number of cell deaths before day 4 of differentiation; too dense will result in reduced homogeneity during the differentiation process; the recommended seeding density is 4.
    0x10
    4 cells/cm 2

    .

    In the differentiation stage of DE-hepatocytes , too sparse initial DE cells will result in a shortened survival and culture cycle and poor performance of the obtained hepatocytes, because the survival and function maintenance of hepatocytes require cell-to-cell contact; if it is too dense, the excess cells will not Will stick to the wall and will be removed when changing the liquid; the recommended inoculation density is 1.
    2x10
    5 cells/cm 2

    .

    2.
    Liquid change time

    During the differentiation stage of iPS-DE cells, change the medium at about the same time every day , try to be from 10 am to 4 pm, to ensure that the cells continue to provide fresh nutrients and growth factors
    .
    In the differentiation stage of DE-hepatocytes, the time for changing the medium is not so demanding , and it can be delayed by 10-12 hours
    .

    3.
    Overlay

    In the differentiation stage of DE-hepatocytes , the preparation, formation and subsequent replacement of the overlay layer (Overlay) are very important
    .

    When changing the liquid on Day 14 and Day 16, make sure that the temperature of the Maturation Medium is between 16°C and 18°C
    .
    If the temperature is too high, the covering layer will gel and fall off before contacting the cells, and a complete covering layer will not be formed; if the temperature is too low, the cells may shrink but still recover

    .

    Do not move and touch the cells within 48 hours of changing the Maturation Medium
    .
    This means that cells should not be observed on Day 15 and Day 17

    .
    Because if the cells are moved, an unevenly distributed covering layer is likely to form, which will significantly affect the subsequent differentiation

    .

    When changing the liquid on Day 18 and the following days, do not damage the covering layer
    .
    We recommend tilting the culture plate, using a manual pipette (single well, multi-well) to carefully remove 90% of the old medium, and carefully add new medium along the side wall, refer to the following diagram

    .

    If you have any questions, please contact us for consultation (Ms.
    Li, 010-80720986,
    lijs@takarabiomed.
    com.
    cn
    )!

    Attached "Takara People iPS-Hepatic Differentiation System (Y30055) Information Collection" , take it away, you are welcome!

    1.
    Operating instructions:
    Cellartis iPS Cell to Hepatocyte Differentiation System User Manual
    2.
    Technical information:
    A simple and complete system to generate mature hepatocytes from various iPS cell sources
    3.
    Technical information:
    Next-generation human iPS cell-derived hepatocytes for metabolic disease modeling
    4.
    Technical information:
    Next-generation human iPS cell-derived hepatocytes for long-term drug metabolism studies
    5.
    Application literature:
    Cellartis iPS Cell to Hepatocyte Differentiation System citation list

    For more details, please click:
    Takara Bio China official website

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