The transcription activation mechanism of GcrA, a new type of transcription factor, is revealed.

Recently, the Chinese Academy of Sciences Center for Excellence in Molecular Plant Science innovation / Plant Physiological Ecology Institute Zhang Yu research team, in collaboration with the MIT Michael Laub research group, with the organization of the institute of the original mechanism of the qiron activation by CaloeproecselicsGCrA, published online in the Nucleic Acids Research, the study revealed the new transcription factor GcrA transcription activation mechanism.
transcription is the main regulatory link of gene expression.
transcription regulation takes various forms, and bacterial transcription regulation is mainly implemented by transcription factors.
they bind specifically to the gene regulatory region, enabling transcription activation or transcription suppression by promoting or inhibiting THE binding of RNA polymerases with regulatory gene promoters.
transcription factor GcrA exists in ???? Deformation bacteria gate, is an important cell cycle regulatory protein, unlike traditional transcription factors, GcrA functions in monomers, can identify the characteristic DNA sequence of methylation, and can be combined with gene transcription upstream or downstream activation transcription.
the researchers analyzed the crystal structure of the compound of GcrA-DBD (DNA binding domain) and methylation DNA, as well as the composite crystal structure of GcrA-CTD and transcription starting factor, the aucryfactor, the aucry, revealed the molecular mechanism of identifying m6A methylation modification, and proposed that GcrA combines DNA with RNAP, i.e. GcrA-CTD, via the co-insinal with the methylated Dna. The interaction of GcrA-DBD with DNA is recruited to the identification site with GcrA regulatory gene, and about 50 amino acid flexible regions between GcrA-DBD and GcrA-CTD enable GcrA to identify the base sequence (-30 upstream, -20 upstream, s10, plus 20; number based on the transcription starting point).
the study proposed a new type of transcription activation mechanism represented by GcrA.
the first author of the paper is Dr. Wu Yuxian Zhang and Diane L. Haakonsen, Ph.D. of the Massachusetts Institute of Technology.
Michael T. Laub and Yu Zhang contributed to this report.
research work has been funded by the National Natural Science Foundation of China project, the Youth Thousand People Program, and the Chinese Academy of Sciences' frontier science research program.
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