-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
- Cosmetic Ingredient
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
This research opens up a new approach to genome editing of wild bacterial species and isolates, such as those with clinical and environmental significance and those that form the human microbiome
background
The CRISPR-Cas system includes the adaptive immune system of prokaryotes, which disarms the virus by cutting the DNA of the invading virus
It is worth noting that CRISPR-Cas systems belonging to different categories and types are constantly being identified, and they provide a deep reservoir for the expansion of CRISPR-based toolkits
Important findings
Previously, the team had identified a highly active IF-type CRISPR-Cas system in a clinically multi-drug resistant strain of Pseudomonas aeruginosa PA154197, which was isolated from a blood infection case in Queen Mary Hospital
In order to overcome the barriers of transferring the complex type I Cascade to a heterologous host, in this study, the research team cloned the entire type I-f cas operon into a vector mini-CTX that is proficient in integration, and coupled (a A common DNA transfer method in nature) transfer the cassette to a heterologous host
In addition, they developed an advanced transferable system, including a highly active IF-type cascade and recombinase, to promote the system in strains with poor homologous recombination ability, and wild Pseudomonas aeruginosa without genome sequence information.
Dr.
Journal Reference :
Zeling Xu, Yanran Li, Huiluo Cao, Meiru Si, Guangming Zhang, Patrick CY Woo, Aixin Yan.