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    Home > Biochemistry News > Biotechnology News > The University of Hong Kong publishes a paper: Editing super bacteria with CRISPR-Cas

    The University of Hong Kong publishes a paper: Editing super bacteria with CRISPR-Cas

    • Last Update: 2021-07-27
    • Source: Internet
    • Author: User
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    This research opens up a new approach to genome editing of wild bacterial species and isolates, such as those with clinical and environmental significance and those that form the human microbiome


    background

    The CRISPR-Cas system includes the adaptive immune system of prokaryotes, which disarms the virus by cutting the DNA of the invading virus


    It is worth noting that CRISPR-Cas systems belonging to different categories and types are constantly being identified, and they provide a deep reservoir for the expansion of CRISPR-based toolkits


    Important findings

    Previously, the team had identified a highly active IF-type CRISPR-Cas system in a clinically multi-drug resistant strain of Pseudomonas aeruginosa PA154197, which was isolated from a blood infection case in Queen Mary Hospital


    In order to overcome the barriers of transferring the complex type I Cascade to a heterologous host, in this study, the research team cloned the entire type I-f cas operon into a vector mini-CTX that is proficient in integration, and coupled (a A common DNA transfer method in nature) transfer the cassette to a heterologous host


    In addition, they developed an advanced transferable system, including a highly active IF-type cascade and recombinase, to promote the system in strains with poor homologous recombination ability, and wild Pseudomonas aeruginosa without genome sequence information.


    Dr.


    Journal Reference :

    1. Zeling Xu, Yanran Li, Huiluo Cao, Meiru Si, Guangming Zhang, Patrick CY Woo, Aixin Yan.



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