 Home > Biochemistry News > Biotechnology News > The Use of Alkaline Phosphatase to Prevent Vector Regeneration

The Use of Alkaline Phosphatase to Prevent Vector Regeneration

 Last Update: 2020-12-14
 Source: Internet
 Author: User

Tags

dna ligase mechanism

dna cloning

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Linearized plasmid vector molecules produced by restriction enzyme digestion can be reformed into circles by the action of
DNA
ligase. This is the most favorable reaction even when foreign DNA fragments are present. Thus, unless a direct selection technique is available, the reformed parental molecule will also give transformants and hence reduce the overall cloning efficiency. Usually most of the recircularized parental plasmids can readily be distinguished from recombinants by the absence of insertional inactivation; nevertheless, this may involve screening large numbers of transformants for each recombinant.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

L-Ascorbate-2-Monophosphate Feed Grade

CAS No.: 109620-90-8

Feed Grade

Potassium Sorbate from reliable manufacturer

CAS No.: 590-00-1

Food Grade

Doxycycline Hydrochloride

CAS No.: 10592-13-9

Pharmaceutical Grade

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.