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Mucins are major differentiated products of mucosal cells throughout the body and are thus important markers of normal and disease development. The use of human colonic cell lines as a model of the adenoma-carcinoma sequence is of particular interest because it allows the changes in expression of mucins to be studied during the development and progression of disease (
1
,
2
). Recently, the importance of proliferation, differentiation, and apoptosis has attracted attention to the use of culture systems for the study of cell behavior in normal and disease processes (
3
,
4
). In the same way, tissue obtained from patients at surgery or as biopsies can be placed in short-term primary or organ culture to study similar changes in disease (
5
,
6
).