The Yichengqi research group of the School of Life Sciences develops a new technology for m6Am modification detection

On August 6, 2021, the Peking University School of Life Sciences and the Peking University-Tsinghua Life Sciences Joint Center Yi Chengqi research group published in Nature Communications the title " m ⁶ Am-seq reveals the dynamic m ⁶ Am methylation in the human transcriptome In the article, a new technology for detecting m ⁶ Am modification on mRNA was developed, and a modification map of N6,2'-O-dimethyladenosine ( m ⁶ Am) in the whole transcriptome was drawn

The dynamic and reversible RNA modification plays a crucial role in the regulation of gene expression

Based on this optimization system and demethylation RNA immunoprecipitation developed m ^.

Figure 1: m ⁶ Am-seq identification of m ⁶ Am and 5'UTR m ⁶ A modifications

(A) m ⁶ Am-seq flow chart; (B) motif analysis of m ⁶ Am; (C) motif analysis of 5'UTR m ⁶ A; (D) m ⁶ Am modification identified by three sequencing technologies Distribution on the transcriptome; (E) Single base detection of m ⁶ Am sites

In summary, the research developed m ⁶ Am-seq, a sequencing technology for m ⁶ Am modification of the whole transcriptome , and obtained credible m ⁶ Am and 5'UTR m ⁶ A modification sites, which are two post-transcriptional The functional study of modification provides a powerful tool .

Professor Yi Chengqi from Peking University School of Life Sciences and Peking University-Tsinghua Life Sciences Joint Center is the corresponding author of this article