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Recently, Researcher Wang Junxin, Dr. Xue Junxin and Associate Researcher Jiang Wei of the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences have made new progress in the study of toxoplasma gondii's pathogenic mechanisms.
the study revealed that thiooxyprotein reduction enzyme (TR) is a toxic factor that plays an important role in toxoplasmosis's resistance to oxidative damage from the host.
research was published online July 7, 2017 in the American Journal of Experimental Biology.
The study successfully identified several important proteins in toxoplasmosis's acute infection serum through immunodeficieric electrophoresis combined with series mass spectrometry, in which toxoplasmosis thiooxyprotein reduction enzyme (TgTR) is a selenium-free cysteine TR that helps pathogens resist oxidative damage from host immune cells by maintaining the reduction of thiooxygen proteins under the conditions of consumption of NADPH.
conducted catalytic dynamics studies on recombinant thiooxyprotein reductases, and used CRISPR/CAS9 gene editing techniques to knock out the toxoplasmosis TR gene, resulting in reactive oxygen (Reactive oxygen species, ROS) and lipid peroxide product propylene dialdehyde (reactive oxygen), which knocked out TgTR. Malondialdehyde, MDA) levels increased, total antioxidant capacity decreased, increased environmental sensitivity to oxidative stress caused by hydrogen peroxide, reduced cell invasion efficiency and proliferation rate, and mice infected with TgTR knockout toxoplasmics survived significantly longer than mice infected with wild or complementary strains.
that TgTR play an important role in toxoplasmia's resistance to oxidative damage and is an important toxic factor in toxoplasmia infection.
TR is the drug target and vaccine candidate antigen molecule of most parasites, and on this basis, further research can provide theoretical basis for the development of toxoplasmic vaccine and the prevention and control of toxoplasmic disease.
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