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    Home > Active Ingredient News > Drugs Articles > This article analyzes the detection principle and test method of Plasmodium P.F/P.V detection kit

    This article analyzes the detection principle and test method of Plasmodium P.F/P.V detection kit

    • Last Update: 2022-10-20
    • Source: Internet
    • Author: User
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    Guangzhou Jianlun Biotechnology Co.
    , Ltd.
    is a collection of research and development, sales, service in one, the company's products involve clinical rapid diagnostic reagents, food safety testing reagents, contraband rapid testing, animal disease epidemic prevention testing reagents, immunodiagnostic reagents, clinical hematology and humoral testing reagents, microbial testing reagents, molecular biology testing reagents, clinical biochemical reagents, organic reagents and many other fields, while the core agent Panbio, FOCUS, Qiagen, IBL, CORTEZ, Fuller, Inbios, BinaxNOW, LumuQuick, Japan Fuji, Japan Bioken and other diagnostic products group company products, is committed to commodity inspection, disease prevention and prevention, anti-drug system, drug rehabilitation center, inspection and quarantine, biochemical enterprises, scientific research institutes, medical institutions and other institutions and industries to provide high-quality products and services
    。 The following small series briefly introduces to you the detection principle and test method of the Plasmodium P.
    F/P.
    V detection kit, as follows: 【Inspection principle】 This product uses the principle of double antibody sandwich method and colloidal gold immunochromatography to qualitatively detect falciparum malaria and vivax malariae antigens in human whole blood, with gold standard P.
    F/P.
    V antibody as an indicator marker, and the detection line 1, detection line 2 and control line on the nitrocellulose membrane are coated with P.
    v antibody, P.
    f antibody and sheep anti-mouse polyantibody
    .
    At the time of detection, the sample is chromatographic
    under capillary effects.
    If the sample under test contains P.
    f/P.
    v antigen, the gold standard P.
    f/P.
    v antibody binds to the P.
    f/P.
    v antigen to form a complex, and binds with the P.
    f/P.
    v antibody fixed at 1/2 of the detection line to form a sandwich during the chromatography process, resulting in a purplish-red band in the detection area (P.
    f/P.
    v); Conversely, no purplish-red bands
    appear in the detection zone (P.
    f) (P.
    v).
    Regardless of the presence or absence of malaria antigen in the sample, the complex continues to be layered upwards into the control zone (C), showing a purplish-red band
    in response to sheep anti-mouse polyantibodies.
    The purple-red band presented in the control area (C) is the criterion for judging whether the chromatography process is normal, and also serves as the internal control standard
    for the reagent.
    【Inspection method】 1.
    Before the test, you must read the instruction manual completely, and restore the test kit and sample to room temperature (20 °C ~ 30 °C)
    before use.
    2.
    Tear open the foil pouch and remove the kit, which should be used
    as soon as possible within 1 hour.
    3.
    Place the kit on a clean and flat countertop, add 1 drop of sample vertically to the wells (S) with a plastic pipette, and then add 2~3 drops of dilution (about 70μL) dropwise into the
    S well.
    4.
    Wait for the fuchsia band to appear, read the result within 15 minutes, and judge it invalid
    after 20 minutes.
    【Interpretation of test results】 Positive (+) ): Fuchsia bands appear in both the detection area (P.
    F) (P.
    v) and the control area (C
    ).
    The results showed that the samples contained both P.
    vivax antigen and Plasmodium falciparum antigen
    .
    If only two fuchsia bands appear
    .
    One is in the detection zone (P.
    F) or (P.
    v) and the other is in the control zone (C).

    The results showed that the samples contained only P.
    vivax antigen or Plasmodium falciparum antigen
    .
    Negative (-)): Only one purplish-red band appears in the control region (C) and no purplish-red band occurs in the detection region (P.
    F
    ) (P.
    v).
    Invalid: No fuchsia bands
    appear in the control area (C).
    Indicates improper handling or the kit has failed
    .
    In this case, you should carefully read the instructions again and retest
    with a new kit.
    If the problem persists, stop using this lot number immediately and contact
    your local supplier.
    Note: Detection zone (P.
    f) (P.
    v)) fuchsia bands can exhibit shades of color
    .
    However, within the specified observation time, even very weak color bands should be judged as positive regardless
    of the color of the color band.
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