This is a winning combination of glycoprotein synthesis

Many processes in the human body are regulated by the function of proteins

The efficacy of enzymes and functional proteins is regulated by protein modification

The production of glycoproteins, such as biology—therapeutics that are produced from living organisms or contain components—use mammalian cell expression methods, but it is impossible to modulate the structure of sugars added to proteins

Researchers at Osaka University have discovered an unprecedented and efficient amide bond formation reaction between glycine-amino acid and two peptides: diacyl disulfide bond coupling and sulfuric acid capture linkage

"We basically use glycosyl asparagine to form a link between two functional peptides to form a glycoprotein," explained Nomura Kota, the first author of the study

The team proved the feasibility of their technology by synthesizing two cytokine glycoproteins

Research corresponding author Yasuhiro Kajihara explained: "We have demonstrated a reliable method for synthesizing glycoproteins, which will allow thorough research on the biological functions of glycoproteins and the production of biologics

DOI

10.

Article title

Glycoprotein semisynthesis by chemical insertion of glycosyl asparagine using a bifunctional thioacid-mediated strategy