Through the genetic analysis of gaoyao1 (gy1), the regulatory mechanism of the gene elongation of rice embryo shaft and germ gyration was identified.

Rice is an important food crop, and its seedlings are mainly composed of root, middle embryo shaft, germ and real leaf.
, the elongation of the middle embryo shaft and the germ crucible promoted the unearthing of rice seedlings.
, it is of great significance to analyze the mechanism of the elongation of the embryo shaft and germ slug for the cultivation of live rice varieties of dry species.
The Zhang Jinsong Research Group and Chen Yuyi Research Group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, in collaboration with the Li Zhikang Research Team of the Chinese Academy of Agricultural Sciences, identified the genes that regulate the elongation of the embryo shaft and germ palate through genetic analysis of gaoyao1 (gy1), a high-waist mutant.
further identified the gene's excellent allegant variant GY1376T in the natural population.
, GY1 is a phospholipidase A1 protein located in chlorogretes that acts as the first step in the biosynthetic synthesis of jasmine acid.
it inhibits the elongation of the mid-embryo shaft and germs by promoting the synthesis of jasmine acid.
functional studies and genetic analysis show that the ethylene signaling pathways mediated by OsEIN2 and OsEIL2 reduce the content of jasmine acid by inhibiting the expression of GY1 and other jasmine synthesis pathway genes, promoting cell elongation, and thus regulating the length of the embryo shaft and germ in rice.
analysis of the variation of the GY1 gene in 3000 rice genomes, and found that the excellent allegant variant GY1376T is different from the gy1 mutation point.
GY1376T is closely related to the long mid-embryo shaft (figure).
: The allegant variation of the GY1 gene GY1376T is associated with the long medium embryo axis.
left: compared to japanese sunny varieties, containing control GY1376G, with short medium embryo shaft and germ, blue fluorescence shows shorter cells.
right: Kasalath variety, containing excellent alleometry variant GY1376T, with long medium embryo shaft and germ crucible, blue fluorescence shows longer cells.
bio-chemical analysis showed that GY1376T had a phospholipase A activity lower than the control GY1 but higher than the mutant gy1 protein.
The conversion of GY1376G, which controls the Japanese sun, into the Kasalath variety with a long mid-embryo shaft and a long germ glyph (containing the allephase variant GY1376T) can promote the synthesis of jasmine acid and inhibit the elongation of the mid-embryo shaft and germ.
study revealed a novel mechanism by which ethylene promotes the elongation of the mid-embryo shaft and germs by inhibiting jasmine acid synthesis during the germination of rice seeds.
In view of the low frequency of all-position variation GY1376T in China's rice varieties, it is of great significance for agricultural production to import the excellent allegant GY1376T into different main-plant varieties.
the study was published online May 2 in the journal Plant Cell (DOI:10.1105/tpc.16.00981).
, Ma Wei and Lu Xiang are co-authors of the paper, and Ma Wei is also a co-author of the communication.
the study was funded by projects such as the 973 program and the National Natural Science Foundation.
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