Tianjin Institute of industrial biology has made new progress in the preparation of healthy sugars from cheap fructose based raw materials

D-alloxan has the characteristics of improving lipid metabolism, reducing postprandial blood sugar and ultra-low calorie It is very similar to sucrose in taste and processing performance It is a new type of "healthy sugar" Jerusalem artichoke is a low-cost non food crop Its tuber is rich in inulin, which is an important fructose based resource Therefore, the preparation of d-alloxan from Jerusalem artichoke has great application potential The team of functional sugars and natural active substances led by sun yuanxia, researcher of Tianjin Institute of industrial biology, Chinese Academy of Sciences, dug out a new type of Exoinulinase from Bacillus Belize, which can realize the efficient hydrolysis of inulin, with the hydrolysis efficiency reaching more than 98% Subsequently, the team developed the efficient preparation technology of the difference isomerase of ectoinulinase and alloxan, established the "one pot two enzyme method" to hydrolyze inulin to produce alloxan, and realized the high added value transformation from the cheap raw material of Jerusalem artichoke to the healthy syrup rich in alloxan This study reduces the production cost of d-alloxan, simplifies the production process, and has a good application prospect The research work is supported by the national key R & D plan and the project of youth innovation promotion association of Chinese Academy of Sciences Relevant research results were published in process Biochemistry (DOI: 10.1016/j.procbio 2019.10.006) Zhu Ping, the graduate student of the research team, and Zeng Yan, the associate researcher, are the first authors of the paper, and Zhu Yueming, the associate researcher, is the corresponding author of the paper