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    Home > Active Ingredient News > Drugs Articles > TLC identification of Schisandra chinensis and its ethanol extract pentanol

    TLC identification of Schisandra chinensis and its ethanol extract pentanol

    • Last Update: 2007-01-31
    • Source: Internet
    • Author: User
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    [Key words] Schisandra chinensis; Wurenchun; TLC; identification of Wuwei given sexual temperature, sour taste, sweet taste, can benefit Qi and promote body fluid, park Shen Ning heart, and its alcohol extraction can significantly reduce serum alanine transaminase, increase bile secretion It can be used for the treatment of various types of hepatitis Since 1990, our factory has used the ethanol extract of wuweiyu in the production of yiganye, a good hepatitis medicine compound It has been identified by TiC for several years It really provides an accurate, fast and simple method for the quality control of our products 1、 The reagents used in the experiment of reagent, silica gel G prefabricated thin-layer plate, silica gel gf254 prefabricated thin-layer plate (Qingdao marine chemical plant) are all analytical pure Schisandra chinensis and Wurenchun are all produced by Wangqing pharmaceutical of our university The standards of schisandrin a, B, C and alcohol B are provided by China Institute of pharmaceutical and biological products 2、 The TLC of Schisandra chinensis was 1.0g from South and North Schisandra chinensis, respectively, adding 3 ml of chloroform, and heating in water bath Reflux 1.5h Filter t filtrate, evaporate the residue, add 1 ml of chloroform to dissolve it as the test solution, take schisandrin, B, alcohol B standard, add chloroform to make 1 mg solution per 1 ml as the control solution, take 2u1 of each of the two solutions and point them on the same silica gel gf254 thin-layer plate, take the upper layer of petroleum ether (30-6o ℃) - hexyl formate formic acid (15:5:1) as the developing agent, and develop The spreading distance is about 8cm, take it out and dry it, put it under 254nm ultraviolet light for inspection, the test sample and the control sample show the same color of fluorescent spots at corresponding positions 2 Identification of Wurenchun extract 0.5g Dissolve with 20 ml ethanol, take the supernatant as the test solution In addition, take schisandrin B, propyl, alcohol B and ethanol to make 1ml solution containing 1mg as standard reference solution Use capillary tube to absorb 10ul reference solution, 2ul reference solution, and then put it on the same silica gel G thin-layer plate Take toluene ethyl acetate (7:3) as developing agent, develop it with a developing distance of 10cm, take out and dry it and spray it with concentrated sulfuric acid solution of chromotropic acid (1g chromotropic acid dissolved in 15ml water, add 15ml) The results showed that there were 30 characteristic spots in the corresponding positions of the test sample and the control sample, and there were 10 round spots corresponding to the turbulence of Schisandra B in rfo.74 At rf0.84, there were 10 circles of yellow purple spots corresponding to schisandrin C, and at rf0.34, there was a circle of dirty green spots corresponding to schisandrin B Discussion and conclusion: 1 The developing agent under the identification of quintanol was tried to use 6:4, 8:2, but the separation effect was not ideal 2 In this paper, Schisandra chinensis and Wurenchun are identified by TLC This method can not only identify the similarities and differences in the origin and quality of wuganzi and Wurenchun, but also overcome the disadvantages of only rational judgment, which provides a reliable guarantee for our factory to produce products.
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