echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Tomato SlDML2 regulates the regulatory mechanism of DNA de methylation.

    Tomato SlDML2 regulates the regulatory mechanism of DNA de methylation.

    • Last Update: 2020-09-01
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    On May 15th, the Proceedings of the National Academy of Sciences (PNAS) published online a research paper by Zhu Health Research Group and LangBo Research Group of the Center for Plant Adversity Biology of the Shanghai Institute of Life Sciences of the Chinese Academy of Sciences entitled "Critical roles of DNA demethylation in the activation of the activation of the ripening-induced genes and the edition of the ripening-repressed genes in tomato."
    The study used CRISPR/Cas 9 technology to obtain mutant plants of tomato sldml2, and found that THE DNA de methylation regulated by tomato SlDML2 not only activates the genes needed for maturation, but also inhibits genes that are not needed for ripening, playing an important role in regulating the ripening of tomato fruit.
    DNA methylation is a conservative egegeneous marker that has an important regulatory role in biological development and environmental response.
    THE DNA methylation and the DNA weathylation process with which it is antagonized are balanced, dynamically regulating the genomic DNA methylation pattern, and the disorder on 2nd side can lead to the disorder of the DNA methylation pattern.
    little is known about the correlation and regulatory mechanism between DNA methylation and fruit ripening.
    studies have shown that DNA methylation may be involved in the ripening of tomato fruits.
    in the process of tomato fruit ripening, DNA methylation level is significantly reduced, while the application of DNA methylation inhibitors can speed up fruit ripening.
    but the specific mechanism of DNA methylation to regulate fruit ripening has not yet been confirmed.
    the study, researchers used CRISPR/Cas 9 technology to obtain mutants in tomato sldml2, a gene that is highly similar to the DNA decmethylase gene ROS1 in amoeba.
    because of the insanity of the gene, the level of methylation in the whole genome is raised, and the gene expression that induces the ripening of the fruit is inhibited, resulting in the fruit of the tomato not ripening properly.
    further research shows that SlDML2 is involved in the activation and expression of mature-related genes, mainly in pigmentation and taste formation, ethylene biosynthesis and signaling pathways, cell wall hydrolysis and other pathways.
    addition, to the researchers' surprise, SlDML2-mediated DNA de methylation inhibits the expression of genes that are not needed in fruit maturation, most of which are involved in photosynthesis and cell wall synthesis.
    the study not only discovered the DNA decethylation function of the SIDML2 gene, but also revealed the important role of DNA decethylation in the mature development of the fruit, which is likely to regulate the accuracy of the ripening of the fruit.
    discovery reveals that DNA methylation may be the third most important regulatory fruit ripening factor in addition to transcription factors and phyto hormones, and has important theoretical and applied value.
    the work was carried out by the Center for Adversity and Purdue University, with Lang bo of the Center for Adversity as the first author and Zhu Health and LangBo as co-authors.
    work has been funded by the Chinese Academy of Sciences and other funds.
    .
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.