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    Home > Biochemistry News > Biotechnology News > Trans-injured synthetic DNA polymerase Pol 457th sucheride can produce oxygen-to-glycosylation modification (O-GlcNAcyation)

    Trans-injured synthetic DNA polymerase Pol 457th sucheride can produce oxygen-to-glycosylation modification (O-GlcNAcyation)

    • Last Update: 2020-08-18
    • Source: Internet
    • Author: User
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    DNA is always attacked by a variety of damage factors in the endogenous or exogenous environment, such as DNA replication errors, cell metabolites, ionizing radiation, ultraviolet radiation, and chemotherapy reagents, all of which can cause DNA damage.
    failure to repair DNA damage in a timely manner will lead to genomic instability, which in turn can lead to a variety of human diseases, such as tumors, neurodegenerative diseases and birth defects.
    organisms have evolved a protective mechanism to monitor DNA damage and repair it in a timely manner, which is the response to DNA damage.
    , the Guo Caixia Research Group of the Beijing Genomics Institute of the Chinese Academy of Sciences, in collaboration with the Tang Tieshan Research Group of the Institute of Zoology of the Chinese Academy of Sciences, found through mass spectrometry that a new protein translation modification can occur across the injured synthetic DNA polymerase Pol,457 sucone: o-GlcNAscolymodification modification.
    known to be exposed to chemotherapy reagents such as UV radiation or cisplatin, the trans-injured synthetic DNA polymerase Pol, was recruited to replace the high-fidelity DNA replication enzyme at the replication fork, and the correct nucleotides were integrated on the opposite side of the corresponding damaged DNA template, thus facilitating the continuation of the replication fork.
    but compared with the high-fidelity DNA replication enzyme, the error rate of the non-damaged DNA template of Pol-discopy increased significantly (10-2 to 10-3), which can easily lead to the inability of genetic information to be properly transferred from the progenitor cells to the child cells, so its recruitment and removal to the replication fork must be strictly regulated, however it is not clear how Pol-din is removed from the replication fork after the completion of THE TLS.
    studies have found that the oxygen-to-glycosine modification that interferes with Polium does not affect its ability to be recruited to the blocked replication fork and to integrate nucleotides on the opposite side of the damaged DNA template, but significantly impairs the interaction between Polium and CRL4CDT2 E3 unophine connective, reducing the 462nd position Lysine's polyunosinmodification levels inhibit the process of separation of Pol-sac and replication forks mediated by the p97-UFD1-NPL4 complex, resulting in increased intracellular mutation rates, increased cell sensitivity to ULTRAviolet and cisplatin reagents, and slow ingenuity of DNA replication fork movement.
    the study revealed the intercoscity between pol-oxygen cosylyged modification and ubiquitin modification, as well as the molecular mechanisms of multiple DNA polymerase conversions in DNA replication.
    significantly increased expression in a variety of tumor cells, closely related to the drug resistance of chemotherapy drugs such as cisplatin, and negatively related to the survival of patients with non-small cell lung cancer.
    the discovery is the first to report that oxygen-gluon-to-glycosine modification is involved in regulating the trans-injury synthesis process and maintaining genomic stability, revealing the molecular mechanism of oxygen-to-glycosine modification sensitive to nutrient levels to regulate genomic stability and tumor resistance from the perspective of DNA damage response, and providing new ideas and strategies to solve the resistance of chemotherapy drugs such as cisplatin, which is expected to improve the survival of some tumor patients.
    research, based on the pol-o-GlcNAcy governs the sires simr cynlluniavia DNA, published online in Nature Communications.
    research work has been supported by the National Natural Science Foundation of China, the Ministry of Science and Technology, etc.
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