echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Plant Extracts News > Transformation of Maize by Electroporation of Embryos

    Transformation of Maize by Electroporation of Embryos

    • Last Update: 2020-11-21
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com


    Plant cell walls present a barrier to
    DNA
    uptake not present in mammalian cells or plant protoplasts. Transformation methods that force DNA through this network of cellulose fibers include particle gun technology (ballistics;
    1
    ), imbibition of dried seeds (
    2
    ), and use of silicon carbide “whiskers” (
    3
    ). Each of these methods has associated limitations. Ballistics, the bombarding of cells or tissues with high-velocity, DNA-coated microprojectiles, has been used most widely and successfully with both cell cultures (
    4

    6
    ) and embryos serving as target tissues. Electroporation has several advantages over ballistics in that it does not require the expensive particle gun apparatus, associated consumable supplies, and licensing. We describe in this chapter an electroporation protocol that has been used successfully to introduce DNA into maize embryos and has resulted in the recovery of stably transformed, fertile, transgenic maize plants.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.
    Related Articles

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent Echemi's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.