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    Home > Biochemistry News > Plant Extracts News > Transformation of Rape with Agrobacterium tumeficiens-Based Vectors

    Transformation of Rape with Agrobacterium tumeficiens-Based Vectors

    • Last Update: 2020-11-20
    • Source: Internet
    • Author: User
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    Agrobacterium tumefaciens
    , a soil bacterium that causes crown gall disease on a wide range of dicotyledonous plants, has been used most widely as a vehicle for gene transfer into plants (
    1
    ,
    2
    ). The transferred genes are stably integrated into the genomes of the transgenic plants and are transmitted to progeny as dominant mendelian traits (
    3
    ,
    4
    ). Since the production of auxins and cytokinins coded by oncogenes on T-
    DNA
    is often incompatible with normal plant development, it is often desirable to “disarm” the plasmids by removing the oncogenes and, therefore, allow the transformed plant cells to differentiate in a normal manner, rather than grow in a tumorous fashion.
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