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Transposon Insertional Mutagenesis in Rice

 Last Update: 2021-01-12
 Source: Internet
 Author: User

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reverse dna

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Insertion mutants offer one of the direct ways to relate a gene to its function by employing forward or reverse genetics approaches. Both T-
DNA
and transposon insertional mutants are being produced in several crops, including rice, the first cereal with its complete genome sequenced. Transposons have several advantages over T-DNA including the ability to produce multiple independent insertion lines from individual starter lines, as well as producing revertants by remobilization. With our new gene constructs, and a two-component transposon
iAc
/
Ds
mutagenesis protocol, we have improved both gene trapping and screening efficiencies in rice.

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