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Transposon Tagging with AC/Ds in Arabidopsis

 Last Update: 2020-11-16
 Source: Internet
 Author: User

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Transposon tagging provides an effective method of isolating plant genes by identifying a mutation tagged by a
DNA
insertion, and then using the DNA tag to isolate the mutant gene (reviewed m ref. I ). The first examples of this approach in plants involved transposon tagging in maize and Antirrhinum using endogenous transposons. More recently, maize transposons were shown to be active in a wide range of plant species, and in some of these have facilitated the use of transposon tagging (
2
‐
5
). In Arabidopsis, Actzvator/Dissoclation (AC/ Ds) have been used to isolate recessive loss-of-function mutations (
6
—
8
), dommant gain of function mutations (
9
) and to isolate genes based on then patterns of expression (
10
,
11
).

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