echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Active Ingredient News > Immunology News > Trends in Immunology , Pasteur Institute uses single-cell sequencing to study animal cell-level immune response, laying the foundation for predicting the spread potential of the virus!

    Trends in Immunology , Pasteur Institute uses single-cell sequencing to study animal cell-level immune response, laying the foundation for predicting the spread potential of the virus!

    • Last Update: 2020-07-22
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    Recently, trends in immunology published a review article entitled "taking insert immunity to the single cell level" by Sarah h é L è ne merkling, a French Pasteur Institute, as the corresponding author.at present, people's understanding of insect immunity mainly includes the molecular mechanism and systemic response of pathogen infection.from the early stage, the key molecular mechanism of pathogen recognition has been clarified, and the coordination of immune response at the tissue level has been basically described.however, the organs and specific cell populations involved in the immune response remain elusive.in this study, the authors emphasized the need for a better understanding of insect immune responses at organ and cell population levels.it will not only improve our understanding of cross species tissue or cell-specific immune processes, but also pave the way for the spatial modeling of infection dynamics in host.single cell technology includes a large number of methods for studying genomics, epigenomics, proteomics and metabonomics at the single cell level.in this study, the authors focused on single cell RNA sequencing (scrna SEQ) and single cell transcriptomics.historically, due to many technical reasons, transcriptomics research has generally been carried out on a large number of cells from multiple individuals, multiple tissues or individuals and tissue pools; they are called bulk RNA sequences.therefore, biological variation at multiple levels, including individual, tissue and cellular differences, can be "hidden" in a transcriptome dataset (Fig. 1).single cell sequencing is applicable to any tissue or organ, and new types of immune cells can be found in Insects Based on the transcriptional similarity with existing immune cells, which can not be obtained by traditional methods.the main challenge of insect single cell sequencing is cell preparation and data analysis.single cell sequencing data sets are more complex and multidimensional than classical RNA sequence data sets.for example, when an Aedes mosquito bites a person infected with an arbovirus (such as Zika virus), the blood containing the virus is first absorbed by the midgut of the mosquito.the virus initially replicates in midgut cells and then spreads in mosquitoes.finally, the virus reaches the salivary glands and spreads again through a new bite.the exact transmission of the virus in mosquitoes is still elusive. how does the virus spread in haemolymph? Is it carried by blood cells or free circulation? In which organs does the virus replicate and when? Are similar immune pathways triggered in the midgut, fat bodies and salivary glands? Can we predict the transmission potential of a virus based on the host's immune response and the level of the virus in certain organs? The authors believe that these basic problems can be solved at least in part by using single cell technology and infection dynamics model. Figure 1. In the past few decades, RNA sequencing technology has evolved from using a single insect library to a single individual, and then to a single organ. at each step, the transcriptome obtained is an "average" image of gene expression. it reflects several individuals, organs or cell types. only by using single cell sequencing can we get the only cell transcriptome. In order to help the majority of researchers to obtain relevant information more effectively, plant biotechnology PBJ has established a wechat group, plant biotechnology Journal submission and literature related issues, official account number and official account submission will be focused on solving problems in the group, and encourage academic exchanges and academic thinking in the group. in order to ensure a good discussion environment in the group, please add a small wechat first, scan the QR code to add, and then we will invite you to join the group in time. tip: when adding small wechat and after joining the group, please be sure to note the name of school or unit + at the end of PI, we will invite you to join Pi group.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.