Two-way immunodifferation test.

I. Objective requirements
1. Review the basic knowledge of consolidating immunochemistry
2. Mastering the operational steps of immunodifferation
3. Mastering its application in the determination and specificity
antibodies
analysis of anti-
serum The evacuation mesh structure of sugar is conducive to the free migration of large molecules
a suitable proportion of
antigen
antibodies combine to form precipitation; the production of
precipitation blocks the free movement of antigen antibody complexes; and the
precipitation belt forms a specific semi-permeable barrier that blocks the same antigen antibody complex while allowing different molecules to pass through.The characteristics and location of the precipitation line:
1 antigen, antibody specificity and concentration,
2 antigen, the size of antibody molecules
3 diffusion rate
when the antigen has a variety of components, will present multiple precipitation lines to cross-reaction lines, so can be used to check the specificity, purity or concentration of antigens between the similarity, so the application range is wider.

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