Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
Last Update: 2021-02-27
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In nature, enzymes often form multienzyme complexes to enhance their catalytic efficiencies and, �moreover, evolve into geneticallyfused multidomain enzymes. Inspired by a natural fusion cytochrome P450 (P450) containing a monooxygenase domain and a reductasedomain, we have developed a heterotrimeric protein-utilized method to form a multienzyme complex composed of a bacterial P450and its catalytically essential two redox proteins. Three distinct proliferating cell nuclear antigens (PCNAs) from
s, each of which can be separately expressed, spontaneously form a heterotrimer. Fusion to the PCNAs enables complex formationof a bacterial P450 and two redox proteins through the self-assembling of the PCNAs and enhances the activity due to efficientelectron transfer in the complex. This PCNA-mediated multienzyme complex formation will be available for other multienzymereactions.
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