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    Home > Biochemistry News > Biotechnology News > Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae

    Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae

    • Last Update: 2020-11-29
    • Source: Internet
    • Author: User
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    The chromatin immunoprecipitation (ChIP) technique has been used to determine where and under what conditions
    DNA
    bindingproteins associate with specific DNA sequences. Proteins are crosslinked in vivo with formaldehyde, and chromatin is thenisolated and sheared. The protein of interest is then immunoprecipitated and the associated DNA sequences identified via
    PCR
    . Although this technique was originally designed to assay DNA binding proteins, it can also be used to monitor mRNA processingfactors associated with transcription complexes.
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