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The chromatin immunoprecipitation (ChIP) technique has been used to determine where and under what conditions
DNA
bindingproteins associate with specific DNA sequences. Proteins are crosslinked in vivo with formaldehyde, and chromatin is thenisolated and sheared. The protein of interest is then immunoprecipitated and the associated DNA sequences identified via
PCR
. Although this technique was originally designed to assay DNA binding proteins, it can also be used to monitor mRNA processingfactors associated with transcription complexes.