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Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing

 Last Update: 2020-12-12
 Source: Internet
 Author: User

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sds gel electrophoresis

single helix

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Single-strand conformational polymorphism analysis has been used successfully to identify single nucleotide changes withinsequences based on the fact that multidetection enhancement gels will separate molecules based on their conformation ratherthan their size. We have expanded the utility of this technique to analyze easily the alternative splicing of pre-mRNAs containingmultiple mutually exclusive exons of the same size. We have used this technique to study the
Caenorhabditis elegans let-2
gene containing two alternative exons and the
Drosophilia melanogaster Dscam
gene, which contains 12 mutually exclusive exons. The ease and the quantitative nature of this technique should be very useful.

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