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    Home > Biochemistry News > Biotechnology News > Vaccine with "cupping"!

    Vaccine with "cupping"!

    • Last Update: 2022-01-12
    • Source: Internet
    • Author: User
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    Using methods similar to cupping, an international team has developed a new method of delivering DNA molecules to skin cells
    .
    Compared with direct injection of DNA, this new technology increases the delivery efficiency by 100 times

    .
    And this new technology is also expected to provide a convenient and low-cost platform for the development of DNA new crown vaccines and more future therapies

    .
    At present, related research has been published in the latest "Science·Progress" journal

    .

    After entering the 21st century, nucleic acid therapy has developed rapidly, and has shown great potential in the treatment of various diseases and the development of vaccines
    .
    The basic principle of this therapy is that after editing or artificially synthesized DNA or RNA fragments enter the host cell, the host's own organelles are used to guide the synthesis of target proteins to achieve specific functions

    .

    In the new crown epidemic, vaccines based on this principle have already shined
    .
    Take the mRNA vaccine as an example.
    After the mRNA enters the host cell, it will instruct the cell to produce the spike protein fragment of the new coronavirus, thereby stimulating the body's immune response

    .

    In nucleic acid therapy, a key step is transfection, that is, allowing DNA or RNA fragments to pass through the cell membrane to enter the nucleus or cytoplasm of the host cell, respectively
    .
    This step is also a major difficulty in the entire nucleic acid therapy

    .

    If no additional protection mechanism is adopted, even if DNA or RNA fragments are injected into the host’s tissues, it is difficult for them to enter the host cell and can only be rapidly degraded in the extracellular matrix
    .
    Therefore, when developing mRNA vaccines, in order to help mRNA molecules cross the cell membrane, researchers use lipid nanoparticles to encapsulate mRNA

    .
    The transfection of DNA fragments is more difficult.
    At present, the most commonly used auxiliary method is to use electroporation to open the cell membrane, but this method often causes side effects such as inflammation, pain and tissue damage

    .

    Is there a way to transport DNA fragments more safely and conveniently? Recently, a research team from Rutgers University in the United States and GeneOne Life Science, a South Korean biopharmaceutical company, used a technique similar to cupping to successfully achieve this goal
    .

    Cupping occupies a place in traditional medicine in China, the Middle East, and ancient Greece
    .
    The basic principle of cupping therapy is to place a heated jar on the skin to generate negative pressure to promote local blood circulation

    .
    Although the effectiveness of this therapy still lacks rigorous clinical evidence, the local negative pressure generated by this process provides an attractive idea for the realization of transfection: If suction technology is used to form a local negative pressure on the skin, then skin cells It will become relaxed under tension, which makes it easier for nucleic acid molecules to cross the cell membrane

    .

    Based on this idea, the research team tested the effect of using this method to deliver a new coronavirus DNA vaccine candidate in a mouse experiment
    .
    They first injected the new crown vaccine into mice intracutaneously, when the DNA fragments in the vaccine were outside the skin cells

    .
    Afterwards, they connected a vacuum pump with a disposable plastic cup and pumped air on the skin of the mouse's back to form a brief negative pressure

    .

    ▲Schematic diagram of experimental device (picture source: reference [1])

    The results of the experiment are amazing: the DNA fragments in the vaccine successfully entered the skin cells of the mice and caused a strong immune response-in a set of control experiments, the immune response induced by this was directly when the DNA vaccine was injected subcutaneously 100 times
    .
    In addition, compared to traditional methods, this new technology is simple, easy to implement, painless and has no side effects

    .

    ▲After direct injection and a combined suction process, changes in the concentration of neocoronavirus antibodies in mice (picture source: reference [1])

    Professor Lin Hao pointed out that this method provides a simple, cheap, and easy-to-scale platform for laboratory and clinical research and development of nucleic acid-based therapies and vaccines
    .
    Especially for the delivery of DNA vaccines, this method has many advantages compared with traditional methods: "This device is low-cost, simple to use, and easy to charge or replace the battery.
    Medical staff only need a small amount of training to master the use

    .
    In addition, because of this method There is no pain or discomfort, and patient acceptance is very high

    .
    "

    Once the above advantages can be reflected in practical applications, then the advantages of the DNA vaccine itself will also appear
    .
    DNA vaccines can not only elicit a strong cellular immune response, but also do not require cold chain logistics, and can eventually reach remote, poor, resource-poor areas

    .

    Due to its remarkable effect in mouse experiments, human clinical trials of this new technology with GeneOne Life Science's GLDS-5310 new crown DNA vaccine are underway
    .
    Currently, Phase 1 clinical trials have verified the safety and immunogenicity of the technology, and clinical trials have entered Phase 2

    .
    Professor Lin Hao introduced that compared with the nucleic acid vaccines that have been published so far, this method produces a better T cell immune response

    .

    Note: The original text has been deleted

    Reference materials:

    [1] Emran O.
    Lallow et al.
    Novel suction-based in vivo cutaneous DNA transfection platform.
    Science Advances (2021).
    DOI: 10.
    1126/sciadv.
    abj0611

    [2] Combining ancient and modern medicine, scientists use cupping to deliver COVID-19 vaccine in lab tests.
    Retrieved Nov 5, 2021 from https://

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