Vanillic acid

Vanillic acid

Vanillic acid

[Synonym] 4-Hydroxy-3-methoxybenzoic acid, 4-Hydroxy-3-methoxybenzoic acid

【Structural formula】

【Molecular formula and molecular weight】C ₈ H ₈ O ₄ ;168.
15

[Source] Scrophulariaceae plant Picrorhiza scrophularii flora.
Pennell dry rhizome
.

【Properties】White crystals
.

This product is easily soluble in methanol and acetone
.

【Purity Check】

TLC

1.
Thin layer plate silica gel GF254 plate

Developing solvent hexane - ether - acetic acid (5: 5: 0.
1)

Check under UV lamp (254nm)

2.
Thin layer plate silica gel G plate

Developing agent toluene- ethyl acetate - glacial acetic acid (8:4:0.
03)

After checking the color development of 10% sulfuric acid ethanol solution, check under visible light

High performance liquid chromatography

Column HypersilC ₁₈ , 5um (4.
6mm×150mm)

Mobile phase Methanol 0.
02% phosphoric acid solution (9:91), 1ml / min

Detection wavelength 260nm

【Structural identification】290,259,217,205
.

3485 (-OH), 1682 (C=O), 1597, 1523, 1433, 1203, 1111, 1028, 918, 881, 766
.

MS m/z: 168[M] ⁺ , 153, 151, 125, 108, 97
.

¹ H-NMR (DMSO) δ: 12.
45 (1H, br, -COOH), 9.
80 (1H, s, -OH), 7.
45 (1H, d, J=8.
0 Hz, H-6), 7.
44 (1H, s , H-2), 6.
83 (1H, d, J=8.
0 Hz, H-5), 3.
79 (3H, s, -OCH ₃ )
.

¹³ C-NMR (DMSO) δ: 121.
6(C-1), 115.
0(C-2), 147.
1(C-3), 151.
1(C-4), 112.
0(C-5), 123.
5(C-6) , 167.
0 (-COOH), 55.
6 (-OCH ₃ )
.

【Storage】Dry and airtight
.