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    Home > Biochemistry News > Plant Extracts News > Virus-Induced Gene Silencing in Plant Roots

    Virus-Induced Gene Silencing in Plant Roots

    • Last Update: 2021-03-16
    • Source: Internet
    • Author: User
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    Virus-induced gene silencing (VIGS) has been used as a quick and easy tool to assess gene function in plants. The virus, carrying a portion of an endogenous gene, triggers a homology-based defense mechanism when introduced into plants. Infection with the modified virus results in gene-specific transcript degradation. Several virus-based vectors have been developed for use in VIGS. One of these virus vectors, tobacco rattle virus (TRV), has a large host range and is transmitted by phytopathogenic nematodes of the genera
    Trichodorus
    and
    Paratrichodorus
    . TRV has been used in VIGS to study gene function in above-ground parts of plants. This chapter describes a protocol for the use of TRV vector to silence genes in roots.
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