Wedelia lactone

Wedelia lactone

Wedelolactone

【Structural formula】

[Molecular formula and molecular weight] C ₁₆ H ₁₀ O ₇；314.

[Source] The dry aerial part of Eclipta prostrata L.

【Properties】Brown needle-like crystals

This product is easily soluble in methanol and ethanol , but almost insoluble in ether

Melting point: 327～328℃

【Purity Check】

TLC

1.

Developing solvent of chloroform - acetone - carboxylic acid (11:6:l)

Inspection by UV lamp at 254nm; spray with ferric chloride test solution and inspect under sunlight

2.

Developing solvent toluene - acetone - formic acid (11: 6:0.

Inspection by UV lamp at 254nm; spray with ferric chloride test solution and inspect under sunlight

High performance liquid chromatography

Chromatographic column Agilent C ₁₈ , 5um (4.

Mobile phase 0-10min methanol- 0.

Detection wavelength 250nm

Differential calorimetry

Start temperature 50℃, end temperature 300℃, heating rate 5℃/min

【Structural identification】350,303,250

3302(-OH), 1719, 1634(C=O), 1446, 1316, 1204, 1154, 1071, 819

MS m/z: 314[M] ⁺

¹ H-NMR (DMSO) δ: 7.

¹³ C-NMR (DMSO) δ: 162.

【Storage】Dry and airtight
.