Wei Wensheng's research group from the School of Life Sciences reported the upgraded version of RNA editing technology "LEAPER 2.0"

RNA editing is a gene editing technology that has emerged in recent years

On February 10, 2022, Wei Wensheng's group published a long research paper " Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo " in the journal Nature Biotechnology .

Circular RNA has no 5' or 3' end, can avoid exonuclease cleavage, and has better stability and longer half-life in cells than linear RNA

Figure 1 Circ-arRNA achieves efficient and precise editing on endogenous transcripts

Since the substrate of ADAR protein is double-stranded RNA, the adenylate in the double-stranded region formed by targeting RNA and circ-arRNA will have different probability of deamination risk

Figure 2 LEAPER2.

The next evaluation of the application potential of LEAPER 2.

Doctoral student Yi Zongyi (CLS), postdoctoral fellow Quan Liang and doctoral student Tang Huixian (BIOPIC) of Wei Wensheng's group are the co-first authors of the paper