What are the key points that affect the pathogenicity and stability of insect-borne yellow virus?

Recently, led by Rao Zi and the team of the Institute of Biophysics of the Chinese Academy of Sciences, the Institute of Microbiological Epidemiology of the Academy of Military Medical Sciences, Oxford University, Beijing Science and Technology Biologics Co., Ltd. and other units jointly analyzed the fine three-dimensional structure of the Japanese Encephalitis virus, an important member of the insect-borne yellow virus, and discovered a series of key locations affecting the pathogenicity and stability of the virus.
insect-borne yellow virus is a class of single-stranded positive-chain RNA viruses with envelopes, including Zika virus, dengue virus, Japanese encephalitis virus, yellow fever virus and other important members, mainly through blood-sucking arthropods (mosquitoes, ticks, white storks, etc.) bite transmission.
, especially in recent years, outbreaks and epidemics of insect-borne viral diseases have become increasingly frequent, seriously threatening human health.
can cause a series of serious diseases such as haemorrhagic fever and encephalitis after infection with human beings, but the molecular mechanisms that lead to different clinical manifestations are not clear.
researchers analyzed the three-dimensional structure of near-atomic resolution of Japanese encephalitis virus particles using cryogenic cryoentherapy technology, the third worm-borne yellow virus structure to be parsed after dengue and Zika viruses.
Compared to the structure of dengue and Zika viruses, the greatest feature of the structure of the Japanese encephalitis virus is the presence of two distinct "holes" on the surface of its envelope E protein congener diomer, and it is particularly noteworthy that there are seven specific motifs in the genome of the insect-borne yellow virus that can cause diseases such as encephalitis, five of which are located near the "holes", suggesting that the above-mentioned specific sequences and structures may play an important role in the onset of encephalitis.
Researchers further used reverse genetics techniques to find that japanese encephalitis virus E protein 107, 138 and 315 amino acids are the key points to determine the neurotoxicity of mice, can play a regulatory role at different stages of the virus replication cycle;
the above findings enrich the understanding of the life cycle and pathogenic mechanism of insect-borne yellow virus replication, and provide new targets and directions for the development of antiviral drugs and vaccines.
, a researcher at the Institute of Biophysics of the Chinese Academy of Sciences, Wang Xiangxi, Qin Chengfeng, a researcher at the Institute of Microbiology Epidemiology of the Academy of Military Medical Sciences, and David Stuart, a professor at the University of Oxford, are co-authors of the paper.
Xiangxi of the Institute of Biophysics of the Chinese Academy of Sciences, Li Shihua of the Institute of Microbiology Epidemiology of the Academy of Military Medical Sciences, And Qinggong, Zhu Ling of Oxford University, are the first authors of the paper.
research has received strong support from the leading projects of the Chinese Academy of Sciences, "Super Molecular Complex", "973" project, the 100-person plan of the Chinese Academy of Sciences, the national key research and development plan, the Newton Senior Scholars Fund and the National Natural Science Foundation.
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