What is the "versatility" of a portable microplate reader?

Portable microplate readers can be widely used in clinical, biotechnology research and pharmaceutical fields, and their onboard data analysis capabilities far exceed the functions
of many computer software suites in terms of the type of fit curve, threshold value calculation, data conversion and verification.
In addition, portable microplate reader data analysis software is used in conjunction with better functionality, including kinetic detection and pore scanning, which can provide powerful data operation functions and data output functions
.
The microplate reader binds the antigen or antibody to the surface of a solid-phase carrier and maintains its immune activity, so that the antigen or antibody is linked to an enzyme to form an enzyme-labeled antigen or antibody, and this enzyme-labeled antigen or antibody retains both its immune activity and the activity
of the enzyme.
During the assay, the sample under test and the enzyme-label antigen or antibody react
with the antigen or antibody on the surface of the solid phase carrier in different steps.
The antigen-antibody complex formed on the solid phase carrier is separated from other substances by washing method, and the amount of enzyme bound to the solid phase carrier is in a certain proportion
to the amount of the substance under test in the specimen.
After the substrate of the microplate reader reaction is added, the substrate is catalyzed by the enzyme into a colored product, and the amount of the product is directly related to the amount of the substance under test in the specimen, so it can be qualitatively or quantitatively analyzed
according to the shade of the color reaction.
Due to the high catalytic frequency of enzymes, the reaction effect can be magnified, so that the assay method can achieve high sensitivity, which can be used in proteomics, genomics, drug screening, environmental testing, various inspection and quarantine fields, microplate reader can carry out quantitative detection of nucleic acid proteins, enzymatic detection, receptor binding research, molecular interaction detection, etc.
, which can be well photometric, fluorescence, luminescence and detection
.
Suitable for laboratories of all sizes, the portable microplate reader enables comprehensive data analysis and fast assay setup, while its large touchscreen makes operation easier
.
It also features a 2-channel dispenser option for unattended carrier racks
for microplate unattended processing in feed units at 20 and 50 positions.
Microplate readers have been coveted in cell detection, enzymatic testing and fluorescence/luminescence report detection
.
The portable microplate reader first adsorbs the antigenic substance in the sample tray, and then adds the sample to be tested to the sample tray, so that the unknown antibody (or antigen) to be tested is combined with the coated antigen (or antibody) to become an antibody antigen complex
.
When the enzyme conjugate and the substrate of the enzyme are added sequentially, a coloring reaction occurs, and the degree of coloration of the microplate reader is related to the amount of antibody to be tested in the sample to be tested, from which qualitative or quantitative detection results
can be obtained.