What precautions are in the face of the source of the co2 culture tank.

what precautions are in the face of the source of carbon dioxide incubators
the choice of 1, CO2 incubators:the manufacturers of CO2 incubators have designed a variety of different devices to reduce and prevent the occurrence of pollution, mainly the anti-pollution design and disinfection system improvements, in order to achieve the purpose of sterilizationSuch as carbon dioxide incubators with ULTRAviolet disinfection function, carbon dioxide incubators with HEPA filters, and carbon dioxide incubators with high temperature disinfection, which are divided into high temperature dry heat and high temperature wet heatThe sterilization capacity of these devices has their own twists and turns, the scope of disinfection of ultraviolet lamps is limited, the farther away from the lamp, the worse the disinfection capacityhePA filters have limited filter membrane apertures, do not remove viruses and some tiny bacteria, and are easy to lose, as well as their limitationsIn contrast, high temperature disinfection is a more effective methodThe high temperature dry heat and high temperature wet heat are also different, because the steam latent heat, strong penetration, easy to make protein denatured or solidified, so the high temperature and humidheat sterilization efficiency is higher than dry heat sterilization methodand improve quality:human factors and attitudes can have a positive or negative impact on the environment, people and thingsFor safety purposes, staff in the laboratory should understand the importance of their attitudes and human factors to the experimental environmentAs a result, laboratory staff are trained3, focus on management:cell chamber co-
incubator should be managed by a person, regularly correct the system, at least once a year Once the values are fixed, do not adjust at will, so as not to affect the temperature, humidity, CO2 concentration fluctuations in the box, reduce the sensitivity of the machine, but also affect the carbon dioxide culture tank in the cell production status Before use, the indicators of the CO2 incubator are systematically corrected, and the box and the interior are disinfected, and the values are stable for a period of time before they can be used In the event of an emergency problem, it should be reported in a timely manner, and should be operated by professional personnel to take immediate countermeasures to avoid unnecessary losses 4
, specification operation: the use of CO2 incubators, although simple, but also need to regulate the operation Entering the cell room must be dressed in a lab suit (white coat), changed into slippers or a disposable shoe cover One-time gloves must be brought in during the experiment, especially before opening the CO2 incubator, you must first spray the hands with 75% alcohol spray pot and then open the CO2 incubator, and then remove the cell culture bottle or culture plate After looking at the cell culture bottle or culture plate under the microscope and putting it back in the CO2 incubator, spray alcohol to disinfect it two door switches in the CO2 incubator to take (put) the material quickly, not for long, otherwise it will affect the concentration of CO2, temperature and humidity, as well as cause pollution Especially don't forget to close the glass door in the inner layer Remember not to say live to the CO2 incubator when you open the door, breathe or even sneeze 5 , strengthen protection: if the experimenter is sick and carrying germs, should be brought with the hood and then into the cell chamber, the serious illness is not suitable for the experiment Female gay sand should be combed to prevent dander contamination and associated incidents If laboratory conditions permit, each person can wear an experimental cap, gloves and a hood to prevent contamination of the CO2 incubator and the self-protection of the experimenter 6 , focus on maintenance: CO2 incubators are placed in the cell chamber, the room needs to be kept dry, the ambient temperature should not exceed 30 degrees C (recommended: 18 degrees C-30 degrees C) The CO2 incubator is placed away from doors and windows, heating equipment and air-conditioning ducts and cannot be directly exposed to sunlight The tabletops of the CO2 incubators must be horizontally shock-resistant and non-flammable and cannot be placed against walls or similar buildings, with at least 30cm of space at the rear power connecting and wall Once put away it is not suitable to move again .