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In the process of using the biological safety cabinet, various kinds of pollution will inevitably occur.
At this time, we need to clean it.
So how can it be considered as effective cleaning? 1.
First of all, when cleaning up the pollution of Class I and Class II biological safety cabinets, equipment that can independently generate, circulate and neutralize formaldehyde gas should be used
.
Place an appropriate amount of paraformaldehyde (final concentration of 0.
8% in air) in a saucepan above an electric heating plate (controlled outside the safety cabinet)
.
2.
Then put 10% more ammonium bicarbonate than paraformaldehyde into another skillet (controlled outside the safety cabinet)
.
Place the pot on the second heating plate outside the cabinet, connect the electric heating plate to the plug outside the safety cabinet, and apply power
.
If necessary, switch the power plug outside the cabinet to control the operation of the panel
.
If the relative humidity is below 70%, an open container with hot water should be placed in a safety cabinet, and the front closure panel should be sealed with strong tape [such as pipe tape]
.
If there is no closing plate in the front, cover the front opening and exhaust with a large plastic sheet to ensure that gas does not leak into the room
.
At the same time, the through hole for the power cable to pass through the front cover must be sealed with tape
.
Insert the heating plate with the acetal plate and turn on the power
.
After the paraformaldehyde has completely evaporated, unplug the plug, cut off the power supply, and let the biological safety cabinet stand for at least 6 hours
.
A second pot is then inserted into the hot plate and the ammonium bicarbonate is energized to evaporate
.
Then unplug the power plug and open the safety cabinet twice for about 2 seconds each time to circulate the ammonium bicarbonate gas
.
Allow the safety cabinet to sit for 30 minutes before removing the front closure plate (or plastic sheet) and exhaust mask
.
Wipe off residue from safety cabinet surfaces before use
.
At this time, we need to clean it.
So how can it be considered as effective cleaning? 1.
First of all, when cleaning up the pollution of Class I and Class II biological safety cabinets, equipment that can independently generate, circulate and neutralize formaldehyde gas should be used
.
Place an appropriate amount of paraformaldehyde (final concentration of 0.
8% in air) in a saucepan above an electric heating plate (controlled outside the safety cabinet)
.
2.
Then put 10% more ammonium bicarbonate than paraformaldehyde into another skillet (controlled outside the safety cabinet)
.
Place the pot on the second heating plate outside the cabinet, connect the electric heating plate to the plug outside the safety cabinet, and apply power
.
If necessary, switch the power plug outside the cabinet to control the operation of the panel
.
If the relative humidity is below 70%, an open container with hot water should be placed in a safety cabinet, and the front closure panel should be sealed with strong tape [such as pipe tape]
.
If there is no closing plate in the front, cover the front opening and exhaust with a large plastic sheet to ensure that gas does not leak into the room
.
At the same time, the through hole for the power cable to pass through the front cover must be sealed with tape
.
Insert the heating plate with the acetal plate and turn on the power
.
After the paraformaldehyde has completely evaporated, unplug the plug, cut off the power supply, and let the biological safety cabinet stand for at least 6 hours
.
A second pot is then inserted into the hot plate and the ammonium bicarbonate is energized to evaporate
.
Then unplug the power plug and open the safety cabinet twice for about 2 seconds each time to circulate the ammonium bicarbonate gas
.
Allow the safety cabinet to sit for 30 minutes before removing the front closure plate (or plastic sheet) and exhaust mask
.
Wipe off residue from safety cabinet surfaces before use
.
