When fresh air becomes a luxury? ——The application of GC-MS in the detection of breath gas

Recently, the news that "New Delhi's air pollution is serious, people spend 30 yuan to breathe oxygen for 15 minutes" attracted public attention It seems a little absurd, but it is also thought-provoking According to reports, the air pollution in New Delhi, India is severe The PM2.5 value in many places is more than 999 The degree of "surface explosion" is equivalent to 33.2 cigarettes per person a day in the local area The damage to the respiratory tract can be seen In recent years, the topic of haze has always been a hot topic On the one hand, environmental problems are serious, on the other hand, people begin to pay more and more attention to their own health Today, people are more and more aware that their health is closely related to one breath and one breath They begin to buy a large number of anti haze masks Air purifier and fresh air system are also necessary for home decoration Pay attention to every breath of air you can know: every breath can also be used as the basis for disease screening and diagnosis? Before the implementation of chronic respiratory disease prevention and control action, the state affairs yuan issued the opinions of the state affairs yuan on the implementation of healthy China action, which clearly pointed out that the prevention and control of four types of chronic diseases, namely cardiovascular and cerebrovascular diseases, cancer, chronic respiratory diseases and diabetes, need to be strengthened In view of the respiratory system diseases, it is proposed to implement the prevention and control actions of chronic respiratory system diseases, guide the key population to detect diseases in early stage, control risk factors, and prevent the occurrence and development of diseases For respiratory diseases, how to find and treat them as soon as possible has become the direction of relevant research - new non-invasive detection methods - now scientists have brought us a new detection method, which only needs a simple breath to be able to carry out disease screening and provide the basis for diagnosis This is the breath test we are going to introduce today, a noninvasive, simple and quick diagnostic method, which can be used to diagnose respiratory diseases (such as asthma) At present, the Metabonomics Study of trace VOCs and other small molecular metabolites in human alveolar gas has been applied in the early screening and research of lung cancer, gastric cancer, colon cancer, breast Xian cancer, diabetes and other major diseases, and there are also relevant reports abroad Cancer Research UK and Owlstone medical started clinical trials on "finding potential biomarkers from the breath of cancer patients" The very low concentration of VOCs in exhaled air has put forward a great challenge to the sensitivity of experimental equipment (pretreatment enrichment and mass spectrometry analysis) First of all, Owlstone medical cooperates with experts in the field of respiratory group to develop a completely non-invasive respiratory detector, reciva breath sampler (below), which controls the switching time of the sampling pump through software and combines with breath biosy cartridge (breath adsorption tube), so as to effectively enrich the exhaled gas components of the tester After collecting the reciva breath sampler of Owlstone medical, the researchers analyzed and injected the VOCs of the breath through the marketd100-xrthermal desorption instrument, using thermo scientific Gamma GC Orbitrap / MS (high resolution orbital well GC-MS) was used for analysis Through Thermo Scientific Gamma Trace finder 4.1 automatically deconvolutes the data and searches the spectral library, and further confirms the qualitative results of mass spectrometry by combining HRF score and RI In the high-resolution data deconvolution and spectrum retrieval interface breath detection of trace finder 4.1, the important biomarkers are often submerged by complex respiratory matrix interference because of low concentration and weak response signal of mass spectrum As a mass spectrometer detector, Orbitrap is famous for its high resolution and high sensitivity At the same time, the wide linear dynamic range makes the compounds to be tested not lead to qualitative errors due to the change of mass accuracy and ion ratio even when they are in extremely high or very low concentration With the whole flow of analytical instruments, this experiment based on the database of smoking related biomarkers to study the breathing gas of different smoking status people (non-smokers, smokers, smokers), and found that the content of dimethylfuran, toluene, ethylbenzene and other compounds in the breathing gas has a high correlation with smoking behavior Orbitrap high-resolution electrostatic field orbital well gas analysis system combined with breath gas sampler and thermal desorption instrument shows great advantages in the analysis of biomarkers of very low concentration breath gas Although the respiratory biopsy is still in the clinical trial stage, it can be expected in the future Once the respiratory cancer test becomes a reality, research will affect the lives of millions of people Through early cancer screening, it is expected to save hundreds of thousands of lives and save more than 1.5 billion US dollars of related medical costs Between breath and breath, there is our pursuit of healthy life, and there will be our scientific protection Reference: clear biopsy: combining thermal degradation gas chromatography with high resolution mass spectrum for improved sensitivity and selectivity in untargeted breath analysis, Jasper boschmans, Christian cojocariu, Paul silcock, Billy Boyle, Alexander Makarov, Max allsworth can scan the QR code below to obtain the whole industry solution of Symantec, or pay attention to the public Hao of "Symantec chromatography and mass spectrometry China", learn more +