Wuhan University develops nano-hole target sequencing detection methods

This newspaper Wuhan, March 3 (Reporter Hou Linliang) reporter from Wuhan University was informed that: the university formed a joint team of innovative development of nano-hole targeted sequencing testing methods, can greatly improve the virus positive detection rate, and can achieve the same day to detect the new coronavirus and other 10 types of 40 common respiratory viruses and monitor the virus mutation, help crack the clinical suspected cases difficult to diagnose the problem.
according to the introduction, the diagnosis of the new coronavirus in the past depends on qPCR nucleic acid testing, but the method shows a high false negative rate and low sensitivity, the positive detection rate is only 30% to 50%, resulting in clinically highly suspected new coronavirus infection patients or low virus latent "false cure patients" detection of "false negative" phenomenon frequently. In addition, qPCR nucleic acid detection is unable to detect other respiratory viruses with high levels of autumn and winter and similar symptoms to neo-coronavirus at the same time, which pose great challenges to outbreak prevention and control and patient diversion management.
Wuhan University School of Pharmacy Professor Liu Tianxuan, Wuhan University People's Hospital Professor Li Yan, Yu Lixuan, Wuhan Yanxi Medical Laboratory Co., Ltd. General Manager Dr. Fu Ace and other joint team developed nanopore target sequencing detection methods. "Nucleic acid detection is like targeting a virus nucleic acid in a sample with a sniper gun, which may not be able to hit, while the new method is to cast a dozen nets, the probability of capturing viral nucleic acid is greatly increased, and the sequence is read at the same time as the capture." Liu Tianxuan said: "From the receipt of samples to the issuance of results, the whole process is controlled in 6-10 hours", the first time to achieve sequencing within 4 hours of high sensitivity, high accuracy detection of new coronavirus.
In addition, the new detection method can detect 10 other respiratory viruses, including Boca virus, rhinovirus, human pulmonary virus, respiratory syncytial virus, coronavirus, adenovirus, side influenza A virus, influenza A virus, influenza B virus and influenza C virus. This facilitates classification management and quickly determines the treatment plan. According to reports, the detection method also has the advantage of being able to monitor the virus mutation, for the outbreak monitoring to provide interpretation and real-time epidemiological information. The nano-hole sequencing platform required for this technology is not very demanding for laboratories and is suitable for use in laboratories such as hospitals and CDC.