X-Gal blue-and-white spot screening.

X-gal (abbreviated to BCIG) is a galactoside and indole.

also known as 5-Bromo-4-chloro-3-indolyl and beta;-D-galactopyranoside or 5-Bromo-4-chloro-3-indolyl and beta;-D-galactoside, Chinese called 5-bromine-4-chlorine-3-lysium-and-beta;-D-semi-lactose glycoside.

molecular group is H15BrClNO6 with a molecular weight of 408.63, CAS Number 7240-90-6.

X-Gal is a color-showing substrate of the samp;beta;-galactosidase, which produces a blue product under the catalysis of the samp;beta;-semi-lactose glycosidease. It is commonly used for in-place stain detection of semi-lactose glycosidease and blue-white spot screening.

X-gal bought when it was solid

you can make a liquid according to the appendix to Molecular Clone

has a preparation method to refer to:

X-gal solution preparation method X-gal for 5-bromine-4-chlorine-3-pyridine-and-beta;-D semi-lactose glycoside. Dissolve 20 mg/ml of storage liquid made with diamide dissolved X-gal. Stored in a glass tube or polypropylene tube, test tube filled with X-gal solution shall be wrapped in aluminum foil to prevent damage due to light and shall be stored at -20 degrees C. The X-gal solution does not < need to be debacterized > aa href".

that is, 20MG per 1 ML

blue White spot screening is a method of recombination sub-screening, which screens recombinators according to the genetic characteristics of the vector, such asantibiotics gene etc. Many of the vectors used today carry a short segment of > E. coli's >DNA" that contains the regulatory sequence of the samp;beta;-semi-lactose glycosidease gene (lacZ) and the coding information of the first 146amino acids A multi-clone bit point (MCS) is inserted in this coding area, which does not destroy the reading box, but allows a few amino acids to be inserted into the amino end of the samp;beta;-semi-lactose glycosidease without affecting the function, and this vector is suitable for host cells that can encode the C-part sequence of the C-end of the sulphase. Therefore, although neither the host nor the particle-coded fragments are enzymatic, when they exist at the same time, they can form an enzymaticly activeprotein . In this way, the lacZ gene complements the host cells that lack near-manipulative gene segments with prosulbases with complete near-manipulative gene segments, called samp;alpha;-complementary. The LacZ-plus bacteria produced by the complementary and complementary , under the role of the inducing agent IPTG, produce blue bacteria in the presence of the pigment X-Gal and are therefore easy to identify. However, when exogenetic DNA is inserted into the multiclonal bits of the protons, it is almost inevitable that the amino end fragments, which do not have the ability to complement each other, cause bacteria with recombined protons to form white bacteriums. This recombinator screening, also known as blue-white spot screening. For example, screening with blue and white spots, the calcified bacteria plate converted by the connecting product 37 degrees C temperature box upside down culture12-16hr, there are recombinant plasm matter particles of bacteria formed white bacteria.