Xie Xiaobing, Director of the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine: POCT nucleic acid testing has great advantages in clinical application

Poct nucleic acid detection, with its fast, simple and easy to operate characteristics, has been rapidly developed in the new crown epidemic sweeping the world.
, the situation of foreign outbreaks is still developing, inflection point has not yet appeared, although the domestic epidemic is basically controlled, but in the face of epidemic prevention and control still can not be taken lightly.
November 13-14, 2020, the "2020 China POCT Annual Meeting" hosted by the China Medical Device Industry Association On-site Rapid Detection (POCT) Branch and the Chongqing Jiangbei District People's Government was held at the Jinyuan Hotel in Chongqing Century.
The conference with the slogan "Beidou plus 5G, Dreaming China POCT" as the theme, with "precise anti-epidemic, health for all" as the theme, the forum covers on-site rapid detection applications, nanoenzymes and POCT, ultrasound detection, POCT and primary health care, POCT and six major centers and other topics, set up a theme conference, 13 academic sub-forum exchanges and enterprise product exhibition.
14, in the accounting site rapid detection sub-forum, Hunan University of Traditional Chinese Medicine, the first affiliated hospital director of technicians, inspection department director Dr. Xie Xiaobing to "nucleic acid detection POCT application in the clinical discussion" as the topic of speech, arterial network on its wonderful point of view was compiled.
Xie Xiaobing: M.D., Chief Technician, Professor, Master's Tutor, Hunan Province High-level Health Talent "225" Engineering Training Object; Chairman of the Professional Committee of Inspection Medicine, Vice Chairman of the Inspection Professional Committee of Hunan Provincial Medical Association, etc., published nearly 100 academic papers, including 50 first or communication authors, 7 SCI articles, editor-in-chief or editor-in-chief for 12 departments, presided over and participated in the "863" plan, the National Natural Science Foundation of Hunan Province, The Natural Science Foundation of Hunan Province and other 14 scientific research projects; Editorial Board of China Clinical Laboratory Management Electronics magazine, "Clinical Testing" magazine review experts.
new coronavirus or long-term existence, epidemic prevention and control need to be normalized at present, the domestic epidemic is basically stable, while the foreign epidemic is still an uncontrollable situation.
has confirmed a second outbreak abroad since October.
to strictly prevent the import of new crown cases abroad, is still the main problem facing our country.
to prevent the outbreak from rebounding in the country, epidemic prevention and control need to be normalized.
Researchers at Harvard University used data from HCoV-0C43 and HCoV HKU1 in the United States between 2014 and 2019 to model the dynamics of viral transmission.
same time, data on SARS-CoV-2 were integrated into the model, estimating the annual infection rate and highest annual prevalence of SARS-CoV-2 between 2019 and 2025.
new research suggests that the new coronavirus could accompany humans until 2025.
, in order to prevent the outbreak rebound, the country's requirements for the prevention and control of the epidemic continued to escalate, in July, August and September for three consecutive months issued to speed up the improvement of medical institutions new coronavirus nucleic acid detection capacity of the relevant notice.
However, in the traditional nucleic acid testing still faces greater challenges: traditional PCR nucleic acid testing technology process is cumbersome, complex operation, need centralized inspection, PCR laboratory is divided into 3-4 zones, need a separate room, sufficient space, equipped with a variety of equipment, high construction costs, high investment, high qualification requirements for operators, long testing time, can not achieve 4 hours to report.
to achieve rapid and convenient nucleic acid detection has become a common demand, the country has also introduced relevant policies to support the new coronavirus rapid nucleic acid detection products research and development.
Xie believes that in the longer period of time that humans struggle with the new coronavirus, the country needs to be normal in the prevention and control of the epidemic.
outpost defense line not only needs to have rapid nucleic acid detection capability to meet the time-thy-time requirements of test results, the primary health care system also needs to have basic nucleic acid detection capability to establish a national joint defense control mechanism.
explores and explores the method of rapid nucleic acid detection, which is in line with the use of scientific and technological power proposed by national leaders from the strategic level.
POCT nucleic acid detection: fast, convenient and efficient at present, the emergence of a number of rapid nucleic acid detection instruments.
these instruments are very lightweight and convenient, and the site requirements are very low, do not require a dedicated PCR laboratory to complete the operation.
rapid nucleic acid detection using the principle of constant temperature amplification, the reaction speed is very fast.
in terms of amplification time, the amplification time of conventional PCR instrument is at least about 100min, while the amplification time of fast nucleic acid detection analyzer is only about 42min.
if it is a strong positive sample, from the extraction of nucleic acids to amplification, the fastest can reach 15min.
so the rapid nucleic acid detection instrument in a very short period of time can complete the sample nucleic acid extraction, release to the final detection of the formation of the report of the entire process, the test results and conventional PCR instrument consistent with good.
POCT nucleic acid detection applications a wide range of laboratory rapid detection programs compared with conventional PCR laboratory programs, each has different application advantages: mobile PCR square chamber laboratory equipped with molecular nucleic acid extractor and PCR instrument is more suitable for high-volume testing, and rapid nucleic acid detection in the room with biosecurity cabinet can carry out new coronary nucleic acid detection, more suitable for emergency testing.
POCT nucleic acid detection is widely used in clinical applications: in addition to the new coronavirus, POCT nucleic acid detection can also be used for a variety of pathogens such as common respiratory tract, digestive tract infectious diseases and infectious pathogen nucleic acid detection;
So, rapid nucleic acid detector can be applied in large public hospitals emergency and fever clinics, improve the efficiency of patients to get emergency treatment, can also be applied in private hospitals with low traffic to help improve their nucleic acid detection capacity, primary hospitals because of the relatively weak medical basic conditions, limited by various factors, is also a good application scenario for POCT nucleic acid testing.
addition, because nucleic acid testing is not limited to the detection of new coronavirus, but also can be used in hospitals for respiratory pathogens, digestive tract infectious diseases and infectious pathogens, such as the identification of diagnosis.
POCT nucleic acid detection for grass-roots prevention and control is of great significance Xie said that rapid nucleic acid detection for grass-roots prevention and control has a very important significance: rapid nucleic acid detection can help graded diagnosis and treatment, with rapid armed grass-roots nucleic acid detection capacity.
because the basic medical conditions of primary medical institutions are relatively weak, the experimental site is not enough, the nucleic acid test sample size is also small, facing the dilemma of input and output ratio difficult to balance.
Because the rapid nucleic acid detection equipment is small, easy to operate, low cost, not subject to medical conditions and testing environment and many other restrictions, can help these medical basic conditions are relatively weak and do not have the ability to detect the new coronavirus nucleic acid secondary hospitals and county (city, district) level / primary medical institutions quickly equipped with the establishment of simple molecular laboratories, perfectly meet its needs for nucleic acid detection, so as to achieve rapid and effective prevention and control of the epidemic.
Rapid nucleic acid detection equipment can also meet the basic testing needs of the grass-roots, such as the common respiratory tract, digestive tract infectious diseases and infectious pathogens for nucleic acid detection, and even for cancer early screening of individual safety drugs for nucleic acid detection, so that nucleic acid detection capacity has better continuity, thereby helping primary medical institutions to improve the ability to accurate diagnosis and treatment.
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