Xu Huaqiang's research group Science published a paper: The high-resolution structure of the inhibitory AMPK complex in the ATP-bound state

The Xu Huaqiang research group of the Shanghai Institute of Materia Medica of the Chinese Academy of Sciences cooperated with various research groups including the Van Andel Institute (Van Andel Institute) Karsten Melcher and other research groups.

AMPK is the most important energy sensor and regulator in the cell.

As a switch for regulating energy metabolism, the activity of AMPK is affected by cellular energy levels, that is, the levels of adenine nucleotides (AMP, ADP, and ATP)

In order to overcome this problem, the cooperative team screened specific antibodies for inhibitory AMPK to stabilize the entire complex, and used cryo-electron microscopy technology to analyze the cryo-EM structure of the inhibitory AMPK complex in the ATP-bound state with near-atomic resolution

a, Cryo-EM structure diagram of the composite

AMPK, KD and CBM in the fully activated state are all phosphorylated, and AMP and drug agonists increase the stability of β-linker by forming an electrostatic interaction network with the two sides of β-linker

Diagram of AMPK activation and inactivation cycle model

Yan Yan, a 2018 Ph.

　　Article link: https://science.

　　Contribution Department: Xu Huaqiang Research Group