Zhang Boli Li Lanqi praises the new coronavirus complete structure map of how cattle

"I hope you can have a good look." It does give a lot of inspiration to think about the evolution of the new coronavirus. On September 19th, at an academic seminar, Zhang Boli, winner of the National Honorary Title of "People's Hero" and
of the Chinese Academy of Engineering, presented a complete structural map of the new coronavirus.
's this a picture? To win Chamberlain
play CALL for it. More than that, the study was reportedly supported by China's
Shi Yigong, Chinese Academy of Engineering
Li Lanxuan, and was urged by Yan Ning, a professor at Princeton University in the United States, to "don't make excuses and have to finish his thesis in three days".
September 20, a reporter from Science and Technology Daily went to Li Sai, a researcher at Tsinghua University's Center for High-Sophisticated Innovation in Structural Biology, to try to find out.
To the entire virus to do "CT" from the outside to see clearly
September 15, "Cells" online published Li Sai team and Li Lanxuan
team completed a research paper, "the molecular structure of the new coronavirus", they through the frozen electro-mirror fault imaging technology, the new coronavirus to see clearly.
frozen electric mirror, structure analysis, hear these two key words, science and technology powder may feel: has been on fire for many years, what new things, is not to change the material again?
, however, the development of new technology can always make people cry "knowledge barren limits imagination!" "
" we put the new coronavirus under a frozen electron mirror, each rotation of 3 degrees to take a photo, a total of 41 shots, followed by a weight structure. Li Sai told Science and Technology Daily that each rotation must be highly consistent with the last one, and that fine lateral deviations are difficult to achieve high accuracy.
method of this distribution imaging is similar to that of medical CT imaging, with the former using electron diffraction imaging and the latter using X-ray diffraction imaging.
as the saying goes, "knowing people is not knowing." LiSai insisted on analyzing the virus's "heart". The team "hit the hand" inside the virus, passing through the cystic membrane and hitting the RNA and winding structures inside to present the most complete image of the new coronavirus to date.
analysis of 100TB of data to map the new coronavirus "popular face
in 2018, Li Sai, who used to work at Oxford University in the United Kingdom, returned to work at Tsinghua University to set up his own laboratory. Prior to this, Li Sai saw and parsed the more intense level 4 virus, but also let a number of level 3 viruses appear real, including the smelly salad virus, Rift Valley fever virus and so on.
when the new crown virus struck, Lisai couldn't sit still. He contacted Li Lanxuan, who was on the front line of the fight against the epidemic in Wuhan, through Shi Yigong, hoping to obtain an inactivation of the new crown virus for power supply mirror research. Li Lanxuan immediate
ly arranged for the team to dock with Li Sai. Through strict polyformaldehyde inactivation, although the new coronavirus "dead", but can still remain alive. Subsequently, the inactivation virus went through rigorous procedures into the laboratory of Tsinghua University.
the virus strain, Li and his team worked more than 120 hours a week. In April, the team successfully collected two batches of 100TB of high-quality frozen electro-mirror fault image data.
are the most core features of a virus that has been infected with thousands of viruses? When the research enters the stage of computational analysis, that is, obtaining the high-resolution structure by the sub-fault image average method, the core features must be assembled by refactoring the virus in order to draw the new coronavirus "popular face".
of the 2,300 viruses in the world, which hedgehog proteins are the most representative? Lisai and his team began manually picking, a highly "artisan" job, based on researchers' past experience with other virus studies, and 50,000 pyrethrins on the surface of the virus were selected for the next protein analysis.
comprehensive analysis of the whole and local, the "sly" nature of the new coronavirus revealed no doubt - the surface prick protein is very small, an average of less than 30, but can freely wander, rotate.
" is the first time such a cystic membrane virus has been seen in a human cognition. The characteristics of protein rotation allow the new coronavirus to freely adjust its position and bind to the subject when attacking cells, Lisse said. See the wind makes the rudder"'s characteristics make it highly contagious.
Locked Skeleton Protein Strategy for Cracking Virus Attacks Hosts
Genetic material determines the sexuality of living organisms, and coronavirus has the longest RNA of any known RNA virus, with nearly 30,000 nucleotides, so how is it done? The Lisse team set its sights on the skeleton protein that collects RNA.
"no one has studied these areas before." 'There is no possible expectation or reference for reshaping the CYP complex (RNP), ' Mr. Lisse said. He took his students to "treasure hunts" out of 20,000 KERNs. "Most of them need to be picked by me because we know too little about internal proteins, and because they're 'crowded like a bunch of grapes' in the virus, they have to be distinguished with high precision so that they don't trap parts of other proteins by mistake." Lisse said.
the cystic protein group of the new coronavirus is becoming clearer, showing the structure of the "egg in the nest". In the "old nest" of the new coronavirus, the ultra-long RNA is densely entangled, and the protein provides the RNA with a super-rule-like skeleton, which is arranged by the "bird's nest" of the hexamerum membrane and "pyramid-shaped" in the center of the globular body. In this way, the new coronavirus can not only be 100 times the length of nucleic acids into their own bodies, but also to meet the vagaries of external challenges.
arrangement of the nucleoprotein rule helps to collect extra-long RNA, and when the virus attacks the host, it is able to release the RNA in an orderly manner without any defects. They can evolve into rotating spools when attacking hosts, output at high speeds, and infect new hosts, Lisse said.
global sharing of structural data could help develop more
vaccines, and little is known about the new coronavirus. Because of this, academia often has different answers to the same questions. For example, regarding the inhibition of a new coronavirus by a medium antibody named CR3022, in March, Science published a paper that found it to have a strong ability to meso- and other teams later found that CR3022 lacked a meso-effect on live viruses.
why are the findings contradictory? It turns out that the former is using in-body recombined viral proteins, while the latter uses live viruses.
" high-precision complete structure of the new coronavirus shows that its surface proteins 'change faces': sometimes they are exposed to antigens, sometimes they are hidden, and sometimes they are in a state of fusion with the membrane. In-body recombination can reveal its law of action only by imitating its 'changed face'. Lisse said.
team's research also corrected and supplemented the previous research. For example, in the first frozen electro-mirror photo of the new coronavirus, released in March this year, the virus's python protein is almost entirely needle-like.
"This may be due to the inactivation of the virus itself destroyed the protein structure of the virus itself, so that its S1 sub-base shedding, S2 sub-base has occurred to the membrane fusion state of the configuration change." Lisse said.
high-precision virus complete structure map helps humans develop more efficient vaccines. "The inactivated methods of the inactivated vaccine now may not leave a 'full body' of the virus's prickly protein, which in theory affects its effectiveness in stimulating the immune system and antibodies." 'These are jobs that can be studied in greater depth, ' Mr. Lisse said.
Lisse team has uploaded the new crown virus HD 3D structure to electron Microscopy Data Bank, a database of structural biology, for free download by scientists around the world to help with popular science education, scientific research, vaccine development and more.