Zhang Feng's latest Cell sub-article: Two new CRISPR-Cas systems.

In a recent paper, a team led by Zhang Feng, a well-known CRISPR research pioneer, used computational sequence database mining to discover two class II CRISPR-Cas systems, subtype VI-B, which lack Cas1 and Cas2, contain a single large-scale effect protein Cas13b, and two previously unsolted related proteins: Csx27 and Csx28.
analysis of the characteristics of these new systems will help develop new tools for manipulating and monitoring cell transcription processes.
The findings were published in the February 16 issue of The Molecular Cell by Professor Zhang Feng of the Broad Institute, who was promoted last year to the youngest tenured professor of Chinese in MIT history, and this year he became the first MIT Professor James and Patricia Poitras, and in the CRISPR patent case, he won a decisive victory in the CRISPR key patent battle: Zhang Feng's team won.
CRISPR-Cas system is an accessive immune system for bacteria that protects microorganisms from foreign nucleic acids through RNA-directed nucleic acid endoenzymes.
although CRISPR has great attention and interest due to its important application in genome editing, CRISPR-Cas systems are also of important and fundamental biological significance.
and notably, the unique mechanisms of these immune systems make them easy-to-use genomic engineering tools.
the most striking aspect of CRISPR-Cas's function, it is arguably the only known example of "adaptive immunity with inheritable genomic memory", the mechanism by which Ramack's adaptive characteristics are inherited.
CRISPR system has a variety of types, of which vi-type CRISPR-Cas system is closely related to microbial immunity and programmed cell biology, and studies show that VI-type effect protein contains two HEPN domains, which are expected to have RNase activity, which requires the activity of two HEPN domains to actually be demonstrated on subtype VI-A effects (Cas13a).
latest article, Zhang Feng's team used a data mining method to discover two sub-types of VI-B CRISPR-Cas systems.
researchers found that these CRISPR-Cas systems were able to achieve iso-expression RNA interference, and through further bio-chemical and genetic experiments, they found that Cas13b used short and long direct repetitive fragments to complete its own CRISPR array, remove the target RNA, and complete RNase activity.
addition, the researchers analyzed the RNA secondary structure required for the target by screening the genes necessary for E. coli to prove that Cas13b has a two-sided pre-region sequence (protospacer-flanking).
at the same time, Csx27 suppresses not Csx28 enhancer, but Cas13b-mediated RNA interference.
description of these CRISPR systems will help develop new tools for manipulating and monitoring cell transcription processes.
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