Abiotic stresses such as salinity and drought are the main environmental factors restricting agricultural production
These stress environments are not conducive to crop growth, resulting in reduced yields and even plant death
Soybean is an important crop, and more than 80% of its demand needs to be imported
If the salt tolerance of soybeans can be improved, on the one hand, it will help enhance the resistance of soybeans to disasters.
On the other hand, it can also use low-salinization land to increase soybean planting area and increase yield
Recently, Zhang Jinsong’s research team discovered a member of the nuclear factor Y ( Nuclear factor Y ) complex, GmNFYA , which is involved in the regulation of histone acetylation modification, thereby promoting the expression of downstream salt-tolerant genes, thereby improving the salt-tolerant capacity of soybeans
There is no difference in the coding region of the GmNFYA gene
among various soybean varieties .
Regardless of whether it is cultivated soybean or wild soybean, when they are exposed to salt stress, the expression of GmNFYA gene will increase significantly, suggesting that soybean salt stress response is closely related to the expression of this gene
Overexpression of GmNFYA gene in the cultivated soybean variety JACK can significantly improve the salt tolerance of genetically modified soybeans (Figure) .
Depth study found, GmNFYA protein capable of histone deacetylase complex component FVE interaction, and FVE with HDA13 (histone deacetylases) interaction .
GmNFYA can compete with HDA13 for binding to FVE protein .
Reducing the expression of FVE and HDA13 genes can improve the salt tolerance of soybean plants .
It is speculated that under normal conditions, the FVE/HDA13 complex deacetylates histone H3K9 , so that nearby salt-tolerant genes are " turned off " .
After salt stress,
GmNFYA accumulates and competitively binds to FVE with HDA13 and releases HDA13 , thereby helping to maintain the acetylation state of nearby histone H3K9 , activating the expression of downstream salt-tolerant genes, and improving soybean salt-tolerance (Figure) .
Further analysis also identified an excellent haplotype I of the GmNFYA promoter .
This haplotype gene is significantly activated by GmNFYA protein under salt stress, so this haplotype can be selected in future breeding practices .
This study reveals the mechanism by which soybean GmNFYA improves salt tolerance by regulating histone acetylation, which has important reference and potential application value for soybean stress tolerance breeding .
The above research was recently published online in Plant Biotechnol J, ( DOI : 10.
Lu Long , a postdoctoral fellow at the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and a staff member, Dr.
Wei Wei, are the co-first authors of this article
Researcher Zhang Jinsong, researcher Chen Shouyi and associate researcher Zhang Wanke are the co-corresponding authors
This research was funded by the key deployment projects of the Chinese Academy of Sciences and the National Natural Science Foundation of China
Figure: GmNFYA improves the salt tolerance of genetically modified soybeans and its mechanism of action
Above : JACK is the control variety, and Null is the negative plant isolated from the offspring of genetically modified soybean